missing translation for 'onlineSavingsMsg'
Learn More

Gata-4, clone: eBioEvan, eBioscience™

Rat Monoclonal Antibody

Brand:  Affymetrix eBioscience 14-9980-80

Code : Z2

Additional Details : Weight : 0.01000kg

 View more versions of this product

Product Code. 15218307

  • £55.52 / 25µg


Estimated Shipment
23-02-2018
Add to basket

Disclaimers:

For Research Use Only.


Description

Description

Description: The eBioEvan monoclonal antibody reacts with mouse and human Gata-4. In a western blot of C57Bl/6 and BALB/c mouse heart lysates, a single band is present, suggesting that this antibody does not cross-react with Gata-5 or Gata-6, which are also expressed in the heart, but with different molecular weights. The eBioEvan monoclonal antibody has been successfully used in flow cytometry on HEK 293T cells transiently transfected with either mouse Gata-4 or human Gata-4. Gata-4 is a member of the GATA family of transcription factors, which are characterized by a conserved DNA binding domain containing two zinc fingers. The mouse Gata-4 gene encodes a protein of 48kDa. Studies show that Gata-4 plays an important role in controlling cardiomyocyte differentiation, proliferation and survival. Gata-4 is expressed during fetal development, as well as in the adult mouse heart. It also appears to modulate gene expression in the gut, liver, and gonads.Applications Reported: This eBioEvan antibody has been reported for use in immunoblotting (WB) and immunohistochemistry.Applications Tested: This eBioEvan antibody has been tested by western blot analysis of mouse heart lysates. As a starting dilution, it is recommended to use this purified antibody at 2μg/mL for western blot analysis. The eBioEvan antibody has been tested on formalin-fixed paraffin embedded human tissue using low pH antigen retrieval and can be used at less than or equal to 5 μg/mL. It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest.Purity: Greater than 90%, as determined by SDS-PAGE.Aggregation: Less than 10%, as determined by HPLC.Filtration: 0.2μm post-manufacturing filtered.
Specifications

Specifications

Gata-4
eBioEvan
Unconjugated
Purified
Rat
25μg
Store at 2-8°C.
Monoclonal
Western Blot
0.5mg/mL
aqueous buffer, 0.09% sodium azide, may contain carrier protein/stabilizer
Gata4
IgG2a
RUO
Primary
Murine
Videos