Invitrogen™ Human GAK, GST Tag Recombinant Protein

Product Code. 15910303

Brand: Invitrogen™ A30973

Description

Recombinant Protein

For maximum recovery please spin prior to use. Unless noted below, aliquots of the 5 μg, 10 μg and 20 μg sizes of kinase are not recommended as materials can be used in original packaging until exhausted. For larger sizes, the number of freeze/thaws may be reduced by preparing aliquots, aliquots below 20 μL are not recommended. Please never store a kinase diluted. If properly stored at -80C, this product is guaranteed for 6 months from date of purchase. This GAK recombinant human protein (1-396) was expressed in insect cells. GAK recombinant human protein is also available in our SelectScreen™ Kinase Profiling Service. Validated for use with the LanthaScreen™ Eu Kinase Binding assay Available in multiple pack sizes for convenient use Also available in our SelectScreen Kinase Profiling Service Protein Form: Recombinant, Catalytic.

GAK serine/threonine kinase or Cyclin-G-associated kinase was originally identified as a protein that is associated with cyclin G. However, neither CDK5 nor GAK kinase activity is activated by cyclin G nor does GAK affect CDK5 kinase activity. GAK seems to act as an auxilin homolog that is involved in the uncoating of clathrin-coated vesicles by Hsc70 in non-neuronal cells. Expression oscillates slightly during the cell cycle, peaking at G1.

Specifications

• Accession Number: O14976
• Concentration: See Label
• For Use With (Application): Kinase Assay
• Formulation: 50mM tris HCl with 10mM glutathione, 0.25mM DTT, 25% glycerol, 150mM NaCl, 0.1mM EDTA, 0.1mM PMSF and no preservative; pH 7.5
• Gene ID (Entrez): 2580
• Molecular Weight (g/mol): 70.2 kDa
• Name: Human GAK, GST Tag
• pH Range: 7.5
• Purification Method: Purified
• Quantity: 10 μg
• Storage Requirements: -80°C, Avoid Freeze/Thaw Cycles
• Regulatory Status: RUO
• Gene Alias: auxilin-2; cyclin G associated kinase; cyclin G-associated kinase; cyclin-G-associated kinase; D130045N16Rik; DNAJ26; DNAJC26; GAK
• Common Name: GAK
• Gene Symbol: GAK
• KinaseFamily: NAK Kinase Family
• Protein Length: 1-396
• Product Type: Protein
• Conjugate: Unconjugated
• Species: Human
• Recombinant: Recombinant
• Protein Tag: GST-tag
• Sequence: 1-396
• Content And Storage: -80°C, Avoid Freeze/Thaw Cycles
• Shipping Condition: Dry Ice
• Expression System: Insect cells
• Protein Family: Kinases & Inhibitors
• Protein Form: Recombinant, Catalytic
• Form: Liquid