GAD1/GAD67 Antibody (GAD1/2563), Alexa Fluor™ 750, Novus Biologicals™

Mouse Monoclonal Antibody

Brand:  Novus Biologicals NBP2-79937AF750

Product Code. 18270117

Description

GAD1/GAD67 Monoclonal specifically detects GAD1/GAD67 in Human samples. It is validated for Western Blot, Flow Cytometry, ELISA.

Specifications

• Antigen: GAD1/GAD67
• Classification: Monoclonal
• Conjugate: Alexa Fluor 750
• Formulation: 50mM Sodium Borate with 0.05% Sodium Azide
• Gene Symbols: GAD1
• Immunogen: Recombinant human GAD1/GAD67 protein fragment (around aa 72-135) (exact sequence is proprietary) (Uniprot: Q99259)
• Quantity: 0.1 mL
• Research Discipline: Diabetes Research
• Test Specificity: This monoclonal antibody recognizes a protein of 67kDa, which is identified as glutamic acid decarboxylase 1 (GDA1). There are two forms of glutamic acid decarboxylases (GADs) that are found in the brain: GAD65 (also known as GAD2) and GAD67 (also known as GAD1. GAD65 and GAD67 are members of the group II decarboxylase family of proteins and are responsible for catalyzing the rate-limiting step in the production of GABA (-aminobutyric acid) from L-glutamic acid. Although both GADs are found in the brain, GAD65 localizes to synaptic vesicle membranes in nerve terminals, while GAD67 is distributed throughout the cell. GAD67 is responsible for the basal levels of GABA synthesis. In the case of a heightened demand for GABA in neurotransmission, GAD65 will transiently activate to assist in GABA production. The loss of GAD65 is detrimental and can impair GABA neurotransmission, however the loss of GAD67 is lethal.
• Content And Storage: Store at 4°C in the dark.

• Applications: Western Blot, Flow Cytometry, ELISA
• Clone: GAD1/2563
• Dilution: Western Blot, Flow Cytometry, ELISA
• Gene Alias: 67kD), EC 4.1.1, FLJ45882, GAD25, glutamate decarboxylase 1 (brain, 67kDa), Glutamate decarboxylase 67 kDa isoform
• Host Species: Mouse
• Purification Method: Protein A or G purified
• Regulatory Status: RUO
• Primary or Secondary: Primary
• Target Species: Human
• Isotype: IgG2b κ