Invitrogen™ Human FYN A, GST Tag Recombinant Protein

Product Code. 15448983

Brand: Invitrogen™ PV6348

Description

Recombinant Protein

For maximum recovery please spin prior to use. Unless noted below, aliquots of the 5 μg, 10 μg and 20 μg sizes of kinase are not recommended as materials can be used in original packaging until exhausted. For larger sizes, the number of freeze/thaws may be reduced by preparing aliquots, aliquots below 20 μL are not recommended. Please never store a kinase diluted. If properly stored at -80C, this product is guaranteed for 6 months from date of purchase. Full-length recombinant human FYN A was expressed by baculovirus in Sf9 insect cells using an N-terminal GST tag. Validated in LanthaScreen™ Kinase Binding Assay Protocols available for simple use and optimized performance Multiple pack sizes available for convenient use Related Product:LanthaScreen™ Eu Kinase Binding Assay Protein Form: Recombinant The gene accession number for FYN A is NP_002028.1.

Fyn A (Fyn isoform A) is a member of the SRC tyrosine kinase oncogene family showing high homology to YES1, FGR and SRC. Fyn has been shown to phosphorylate Dab1, an intracellular adaptor protein that interacts with amyloid precursor protein (APP) and apoE receptor 2 (apoEr2). The interaction of Fyn and Dab1 regulates the phosphorylation, trafficking, and processing of APP and apoEr2. Fyn expression has been shown to be significantly increased in Chronic Myelogenous Leukemia (CML). Knockdown of Fyn with shRNA slows leukemia cell growth, inhibits clonogenicity, and leads to increased sensitivity to imatinib.

Specifications

• Accession Number: P06241
• Concentration: See Label
• For Use With (Application): Kinase Assay
• Formulation: 50mM tris HCl with 0.1mM PMSF, 150mM NaCl, 25% glycerol, 10mM glutathione, 0.1mM EDTA, 0.25mM DTT and no preservative; pH 7.5
• Gene ID (Entrez): 2534
• Molecular Weight (g/mol): 87.1 kDa
• Name: Human FYN A, GST Tag
• pH Range: 7.5
• Purification Method: Purified
• Quantity: 1 mg
• Storage Requirements: -80°C, Avoid Freeze/Thaw Cycles
• Regulatory Status: RUO
• Gene Alias: FYN
• Common Name: FYN Isoform A
• Gene Symbol: FYN
• Protein Length: 1-537
• Product Type: Protein
• Conjugate: Unconjugated
• Species: Human
• Recombinant: Recombinant
• Protein Tag: GST-tag
• Sequence: 1-537
• Content And Storage: -80°C, Avoid Freeze/Thaw Cycles
• Kinase Group: Cytoplasmic Tyrosine Kinase
• Expression System: Baculovirus
• Protein Form: Recombinant
• Form: Liquid