Gibco™ FreeStyle™ F17 Expression Medium

Product Code. 10388033

Brand: Gibco™ A1383501

Description

An animal origin-free, chemically defined, protein-free medium specifically developed to support the growth and transfection of 293-F and CHO cells in suspension without adaptation.

FreeStyle™ F17 Expression Medium is an animal origin–free, chemically defined, protein-free medium specifically developed to support the growth and transfection of 293-F and CHO cells in suspension without adaptation. This is a multi-platform medium compatible with a range of transient expression systems. The addition of 4–8 mM L-glutamine or GlutaMAX™ supplement is required before use. Gibco™ FreeStyle™ F17 Expression Medium features:

Little or no required adaptation

Ability to support suspension growth and transfection of 293-F and CHO cells

Animal origin–free, protein-free, and chemically defined formulation

Scalability in spinner flasks and bioreactors

Little or no required adaptation
Gibco™ 293-F, 293-H, FreeStyle™ 293-F, and CHO-S cells in suspension can be cultured directly into Gibco™ FreeStyle™ F17 Medium without adaptation. Cells adapted to other commercially available serum-free media can be subcultured directly into Gibco™ FreeStyle™ F17 Medium, usually without any further adaptation. Cells usually require adaptation from serum-containing formulations.

Ability to support suspension growth and transfection of 293-F and CHO cells
Gibco™ FreeStyle™ F17 Medium provides equal or better performance compared to Gibco™ FreeStyle™ 293 Expression Medium and Gibco™ FreeStyle™ CHO Medium. Gibco™ FreeStyle™ F17 Medium supports 293-F cells at a peak density of 6–7 × 10⁶ viable cells per mL, and supports CHO-S cells at a peak density of 8–9 × 10⁶ viable cells per mL. Cells cultured in Gibco™ FreeStyle™ F17 can be transfected using a wide variety of transfection reagents, including lipid-based transfection reagents, such as 293fectin™ and Freestyle™ MAX Reagent. Protein expression typically exceeds 75–150μg/mL IgG in 293-F cells.

Animal origin–free, protein-free, and chemically defined formulation
Gibco™ FreeStyle™ F17 Medium is animal origin–free, protein-free, and chemically defined, allowing for easier purification of your protein of interest. Gibco™ chemically defined media contain no proteins, hydrolysates, or components of unknown composition.

Scalability in spinner flasks and bioreactors
Protein production using Gibco™ FreeStyle™ F17 Medium can be scaled up in spinner flasks or bioreactors. The appropriate spinner or impeller speed and seeding density should be optimized for each system. Depending on the impeller design and speed, it may be necessary to supplement Gibco™ FreeStyle™ F17 Medium with additional Pluronic™ F-68 to avoid sheer stress in the culture.

cGMP manufacturing and quality system
Gibco™ FreeStyle™ F17 Medium is manufactured at a cGMP-compliant facility located in Grand Island, New York. The facility is registered with the FDA as a medical device manufacturer and is certified to ISO 13485 standards.

Specifications

• Cell Line: HEK293
• Classification: Animal Origin-free, Chemically-defined, Protein-free, Serum-free
• Concentration: 1 X
• Form: Liquid
• Product Type: Expression Medium
• Serum Level: Serum-free
• Sterility: Sterile
• Without Additives: No Glutamine
• For Use With (Application): Protein expression, viral vector production
• Product Line: Freestyle™
• Quantity: 1 L
• Shipping Condition: Room Temperature
• Endotoxin Level: < 1 EU/mL
• Format: Bottle
• Purity or Quality Grade: Qualified for Further Manufacturing
• Concentration Ratio: 1:1
• Content And Storage: Storage conditions: 2-8°C. Protect from light
  Shipping conditions: Ambient
  Shelf life: 12 months from date of manufacture
• Culture Environment: CO₂
• Culture Type: Suspension Cell Culture

For Research use or Further Manufacturing. Not for diagnostic use or direct administration into humans or animals.