Foxp3 Rat anti-Mouse, Rat, Alexa Fluor 532, Clone: FJK-16s, eBioscience™

Rat Monoclonal Antibody

Brand: Affymetrix eBioscience

Manufacturer Part Number: 58-5773-80


UNSPSC: 12352200

Code: Z2

Additional Details:
Additional Details: Weight: 0.25000kg

Disclaimers: For Research Use Only.

Product Code. 15312180

Quantity Price
1 £101.00 / 25µg
Estimated Shipment date
from Supplier 31-10-2016
Add to basket

Description and Specification


Antigen Foxp3
Applications Flow Cytometry (Intracellular Staining)
Clone FJK-16s
Concentration 0.2mg/mL
Conjugate Alexa Fluor 532
Format Conjugated
Formulation aqueous buffer, 0.09% sodium azide, may contain carrier protein/stabilizer
Gene Alias Forkhead Box P3, Scurfin, JM2
Host Species Rat
Isotype IgG2a
Quantity 25μg
Regulatory Status RUO
Species Reactivity Bovine, Canine, cat, Mouse, Pig, Rat
Storage Requirements Store at 2-8°C. Do not freeze. Light-sensitive material.
Primary or Secondary Primary
Monoclonal or Polyclonal Monoclonal

The FJK-16s antibody reacts with mouse, rat, dog, porcine, bovine and cat Foxp3 also known as FORKHEAD BOX P3, SCURFIN, and JM2; cross reactivity of this antibody to other proteins has not been determined. Foxp3, a 49-55kDa protein, is a member of the forkhead/winged-helix family of transcriptional regulators, and was identified as the gene defective in ‘scurfy’ (sf) mice. Constitutive high expression of foxP3 mRNA has been shown in CD4+CD25+ regulatory T cells (Treg cells), and ectopic expression of foxp3 in CD4+CD25- cells imparts a Treg phenotype in these cells.

Immunoblotting with FJK-16s antibody has mapped the epitope to amino acids 75-125 of the mouse Foxp3 protein. In the human, this region has been shown to be alternatively spliced at the mRNA level. Both the alternatively-spliced and non-spliced isoforms are present in the CD4+CD25+ subset of lymphocytes. Preliminary RT-PCR experiments have not revealed this alternatively-spliced isoform in mouse splenocytes, suggesting different gene regulation in the mouse and human.