Foxp3 Mouse anti-Human, PE, Clone: 236A/E7, eBioscience™

Mouse Monoclonal Antibody

Overview
Brand: Affymetrix eBioscience

Manufacturer Part Number: 12-4777-41

Code: NEW

Additional Details:
Additional Details: Weight: 0.23750kg



Disclaimers: For Research Use Only.

Product Code. 15516986

Quantity Price
1 £ 86.73 / 25 tests
EU Stock 9
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Description and Specification

Specification

Monoclonal or Polyclonal Monoclonal
Gene Alias Forkhead Box P3, Scurfin, JM2
Quantity 25 tests
Applications Flow Cytometry (Intracellular Staining)
Conjugate PE
Host Species Mouse
Format Conjugated
Antigen Foxp3
Species Reactivity Human, Non-human primate, Rhesus Ma
Isotype IgG1
Formulation aqueous buffer, 0.09% sodium azide, may contain carrier protein/stabilizer
Primary or Secondary Primary
Regulatory Status RUO
Storage Requirements Store at 2-8°C. Do not freeze. Light-sensitive material.
Concentration 5μL (0.25μg)/test
Clone 236A/E7

The 236A/E7 antibody reacts with human Foxp3 protein also known as FORKHEAD BOX P3, SCURFIN, and JM2. Foxp3, a 49-55kDa protein, is a member of the forkhead/winged-helix family of transcriptional regulators, and was identified as the gene defective in ‘scurfy’ (sf) mice. Constitutive high expression of Foxp3 mRNA has been shown in CD4+CD25+ regulatory T cells (Treg cells), and ectopic expression of Foxp3 in CD4+CD25- cells imparts a Treg phenotype in these cells.

Intracellular staining and flow cytometric analysis of freshly isolated human peripheral blood mononuclear cells (PBMCs) with the 236A/E7 antibody using the Foxp3/Transcription Factor Staining Buffer Set (cat. 00-5523) and protocol reveals staining of the CD4+CD25bright population.

The epitope from 236A/E7 is different from that of PCH101 (cat. 72-5776). This antibody has also been shown to recognize rhesus macaque, sooty mangabey and cynomolgus macaque.