Foxp3 Mouse anti-Human, eFluor 450, Clone: 236A/E7, eBioscience™

Mouse Monoclonal Antibody

Overview
Brand: Affymetrix eBioscience

Manufacturer Part Number: 48-4777-41

25TEST Anti-Human Foxp3 eFluor 450

Code: NEW

Additional Details:
Additional Details: Weight: 0.23750kg



Disclaimers: For Research Use Only.

Product Code. 15589746

Quantity Price
1 £ 138.8 / 25 tests
Estimated Shipment date
from Supplier 08-12-2016
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Description and Specification

Specification

Antigen Foxp3
Applications Flow Cytometry (Intracellular Staining)
Clone 236A/E7
Concentration 5μL (0.25μg)/test
Conjugate eFluor 450
Format Conjugated
Formulation aqueous buffer, 0.09% sodium azide, may contain carrier protein/stabilizer
Gene Alias Forkhead Box P3, Scurfin, JM2
Host Species Mouse
Isotype IgG1
Quantity 25 tests
Regulatory Status RUO
Species Reactivity Human, Non-human primate, Rhesus Ma
Storage Requirements Store at 2-8°C. Do not freeze. Light-sensitive material.
Primary or Secondary Primary
Monoclonal or Polyclonal Monoclonal

The 236A/E7 antibody reacts with human foxp3 protein also known as FORKHEAD BOX P3, SCURFIN, and JM2; cross reactivity of this antibody to other proteins has not been determined. Foxp3, a 49-55kDa protein, is a member of the forkhead/winged-helix family of transcriptional regulators, and was identified as the gene defective in ‘scurfy’ (sf) mice. Constitutive high expression of Foxp3 mRNA has been shown in CD4+CD25+ regulatory T cells (Treg cells), and ectopic expression of foxp3 in CD4+CD25- cells imparts a Treg phenotype in these cells.

Intracellular staining and flow cytometric analysis of freshly isolated human peripheral blood mononuclear cells (PBMCs) with the 236A/E7 antibody using the Foxp3 Staining Buffer Set (cat. 00-5523) and protocol reveals staining of the CD4+CD25bright population.

The epitope from 236A/E7 is different from that of PCH101 (cat. 72-5776).