Molecular Probes™ FluxOR™ Potassium Ion Channel Assay
FluxOR™ Potassium Ion Channel Assay
Brand: Molecular Probes™ F10017
Code : 50
Additional Details : Weight : 0.00450kg
- K+ channel specific—measure ion flux in both voltage- and ligand-gated potassium channels
- Fast—perform screens in high-throughput mode with reproducible results and excellent S/N without quenching dye
- Pharmacologically relevant—known blockers show dose-dependent inhibition in a large signal window
The FluxOR™ Potassium Ion Channel Assay enables rapid and robust high-throughput screening (HTS) of potassium channel targets in a novel equilibrium measurement, reproducibly giving IC50 values that are predictive of block or modulation in lower-throughput platforms. The FluxOR™ dye is sensitive enough that low mM levels of extracellular thallium give large signals in high-throughput mode. For most applications, FluxOR™ dye is dissolved in physiological HBSS buffer for loading into cells, assisted by our proprietary Powerload™ formulation (Catalog number P10020).
The FluxOR™ Potassium Ion Channel Assay provides a concentrated thallium solution and all necessary buffers, allowing maximum target flexibility and ease of operation in a homogenous format that has been demonstrated for use with cells stably expressing hERG, as well as our BacMam-hERG delivery and expression reagent (Catalog number B10019 and B10033). Optional for use with this kit is the hERG potassium channel cDNA engineered into Invitrogen’s BacMam delivery and expression system. The combination of a bright proprietary fluorescent potassium sensor dye and the hERG potassium channel gene delivered by BacMam affords excellent assay design flexibility and superior sensitivity in detecting potassium channel activity in biologically relevant systems at physiological conditions without the need for quenchers.
|Fluorescence Microscope, Microplate Reader|
|Store in freezer (-5 to -30°C) and protect from light.|
For Research Use Only. Not for use in diagnostic procedures.