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Molecular Probes™ Fluo-4 Direct™ Calcium Assay Kit

Fluo-4 Direct™ Calcium Assay Kit

Brand:  Molecular Probes™ F10472

Code : 50

Additional Details : Weight : 0.00450kg

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Product Code. 10033614

  • £299.00 / Each

In stock, ready to ship: 10

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For Research Use Only. All usage must comply with product instructions.
 

Description

Description

The Fluo-4 Direct™ Calcium Assay Kit was formulated to provide a homogeneous fluorescent calcium assay that:
1. Easily loads into cells
2. Achieves a large assay window and
3. Suppresses background fluorescence generated from the calcium indicator in complete media with little-to-no impact on the specific cellular fluorescence

This advanced formulation allows the assay to be run in a simple addition only format in the presence of serum-containing media. Fluo-4 Direct™ is the third addition to the Fluo-4 family of calcium detection reagents. Fluo-4 AM and Fluo-4 NW both require media removal before assay detection, but Fluo-4 NW adds convenience by including the PowerLoad™ reagent for ease in cell loading. The Fluo-4 Direct™ Calcium Assay Kit is similar to Fluo-4 NW in that it is formulated with PowerLoad™ for ease in cell loading, but unique in that it can be used in the presence of complete culture media and will efficiently suppress background fluorescence without sacrificing the specific cellular fluorescence generated in the assay.
Specifications

Specifications

Cell-Permeant
Visible
Visible
Fluorescent Dye-Based
Chemical
1 x 100mL
Nucleus, Organelles, Cytoplasm
100 ml
Confocal Microscope, Fluorescence Microscope, High Content Instrument, HTS Reader, Microplate Reader, Fluorescent Imager
• 100 ml Fluo-4 Direct™ Calcium Assay Reagent (Component A)
• 2 × 77 mg Probenecid (Component B)
• 200 ml Fluo-4 Direct™ Calcium Assay Buffer (Component C)

Store at ≤-20°C. Dessicate and protect from light.
488⁄530
Powder
Calcium Indicator
Molecular Probes®
Room Temperature
Signal Transduction
Fluorescent
Green
Videos
Documents

For Research Use Only. Not for use in diagnostic procedures.