Molecular Probes™ Flow Cytometry Size Calibration Kit (nonfluorescent microspheres)

Product Code.	Quantity	unitSize
10583473	1 kit	1 set

Product Code. 10583473 Supplier Molecular Probes™ Supplier No. F13838

Description

Flow Cytometry Size Calibration Kit (nonfluorescent microspheres)

Our Flow Cytometry Size Calibration Kit has nonfluorescent particle-size calibration standards that provide a simple, accurate way to determine cell sizes by flow cytometry. The kit contains six suspensions of highly uniform polystyrene microspheres with the following diameters: 1.0μm, 2.0μm, 4.0μm, 6.0μm, 10.0μm and 15.0μm. All of the microsphere suspensions are provided in convenient dropper vials.

View additional information for flow cytometry calibration and size reference beads.

Specifications

• Diameter (Metric): 1, 2, 4, 6, 10, 15
• Content And Storage: Contains six vials of highly uniform polystyrene microspheres: 1.0 μm, 2.0 μm, 4.0 μm, 6.0 μm, 10 μm, 15 μm - diameter microspheres.
  Store in refrigerator at 2°C to 8°C
• Format: Suspension
• For Use With (Equipment): Flow Cytometer
• Quantity: 1 kit
• Detection Method: scatter
• Excitation Wavelength Range: nonfluorescent
• Shipping Condition: Room Temperature
• For Use With (Application): Flow Cytometry
• Product Type: Flow Cytometry Size Calibration Kit

Frequently Asked Questions (FAQs)

What is the Flow Cytometry Size Calibration Kit (Cat. No. F13838) used for?

Our Flow Cytometry Size Calibration Kit has non-fluorescent particle-size calibration standards that provide a simple, accurate way to determine cell sizes by flow cytometry. The kit contains six suspensions of highly uniform polystyrene microspheres with the following diameters: 1.0 µm, 2.0 µm, 4.0 µm, 6.0 µm, 10.0 µm and 15.0 µm. All of the microsphere suspensions are provided in convenient dropper vials. This kit looks at the scatter properties of non-fluorescent microspheres of known sizes to determine approximate cell sizes.

What is the smallest size that I can detect with the Attune NxT Acoustic Focusing Cytometer?

The smallest size that you can detect with the Attune NxT Acoustic Focusing Cytometer is 0.5 µm.

What is the Attune NxT Autosampler?

The Attune NxT Autosampler, an optional accessory for the Attune NxT Acoustic Focusing Cytometer, enables rapid processing of up to 384 samples. It has broad compatibility with different plate formats, both 96- and 384-well plates. It has an intelligent probe designed to minimize clogging and carryover (<0.5%) and to prevent damage to the instrument. It mixes by aspiration rather than shaking to ensure homogeneity of the sample and maintain cell viability. Is performs automated cleaning as part of the shutdown process of the Attune NxT Cytometer. It provides consistent data regardless of sampling method (tube vs. plate) and collection rate.

What are the advantages of acoustic-assisted hydrodynamic focusing in flow cytometry?

-Modular design - Multiple configurations available - field upgradable.
-Save time - 10X faster speeds with no loss in data quality.
-Simplified sample prep - No wash, no lyse options, non-clogging fluidics.
-Enables unique applications - Complex protocols on a broad range of cell types and samples.

How is the Attune NxT Acoustic Focusing Cytometer different from traditional flow cytometers?

With the option to be configured with up to 4 lasers and 14 colors for multi-parameter analysis the Attune NxT Acoustic Focusing Cytometer was designed as a modular system to fit most experimental needs and lab budgets. The novel design of the optical path helps ensure precise fixed alignment of four spatially separated lasers onto the sample stream enabling consistency in data over time, superior performance, and superior reliability. The instrument can be configured with up to 4 solid-state lasers (405 nm, 488 nm, 561 nm, and 637 nm) with flat top beam profiles.

The Attune NxT Flow Cytometer's acoustic focusing uses ultrasonic radiation pressure (> 2 MHz) to transport particles into the center of the sample stream. This pre-focused stream is then injected into the sheath stream, which supplies an additional hydrodynamic pressure to the sample. The combination of these two forces- termed acoustic-assisted hydrodynamic focusing-results in a narrow core stream and uniform laser illumination, regardless of the sample input rate. In traditional cytometers that rely solely on hydrodynamic focusing, the sample core widens to accommodate the increases in flow rate, which results in less uniform laser light illumination.

What is flow cytometry?

Cytometry is the measurement of physical or chemical characteristics of cells or particles. Flow cytometry measures these characteristics of cells or particles as they individually pass lasers in a flow cytometer instrument. Flow cytometry is performed on single cells, providing discrete measurements for each cell in the sample. It also provides a statistical distribution of the measured characteristics of the sample.

A flow cytometer is made up of three subsystems: fluidics, optics, and electronics. Fluidics moves the cells and introduces them for interrogation. Optics generates and collects the light signals. Electronics converts the optical signals to proportional electronic signals for computer analysis.

For Research Use Only. Not for use in diagnostic procedures.