Novus Biologicals™ Flagellin Native Protein

Product Code. 18374624

Brand: Novus Biologicals™ NBP318154

Description

Native Protein

Binds to human & mouse TLR5. Activation of TLR5 in human epithelial cell assays based on NF-kB luciferase fusions, on expression of IL-8 (CXCL8), CCL20, inducible nitric oxide synthase, beta-defensin 2 & matrilysin (MMP-7). Maturation of dendritic cells in in vitro & in vivoassays. Adjuvant activity on foreign antigens delivered by subcutaneous & intranasal routes in mice. TLR5 can be stimulated in vitro by Flagellin 5-50ng/mL & in vivo at a starting concentration of 0.1-1 ug/mouse.)

Specifications

• Accession Number: P06179
• Concentration: LYOPH
• For Use With (Application): Bioactivity, ELISA
• Formulation: Lyophilized from PBS
• Quantity: 100 μg
• Storage Requirements: Store at 4C short term. Aliquot and Store at -20°C long term. Avoid freeze-thaw cycles.
• Endotoxin Concentration: <0.1 EU/μg purified protein (LAL test; Lonza).
• Reconstitution: Reconstitute with 100 ul sterile water (1mg/ml after reconstitution)
• Research Category: Immunology, Innate Immunity
• Purity or Quality Grade: >90% SDS-PAGE
• Protein: Flagellin