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Novus Biologicals™ Flagellin Native Protein

Product Code. 18351133
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Quantity:
10 μg
Unit Size:
10µg
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Product Code. Quantity unitSize
18351133 10 μg 10µg
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Product Code. 18351133 Supplier Novus Biologicals™ Supplier No. NBP318149

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Description

Native Protein

Binds to human & mouse TLR5. Activation of TLR5 in human epithelial cell assays based on NF-kB luciferase fusions, on expression of IL-8 (CXCL8), CCL20, inducible nitric oxide synthase, beta-defensin 2 & matrilysin (MMP-7). Maturation of dendritic cells in in vitro & in vivo assays. Adjuvant activity on foreign antigens delivered by subcutaneous & intranasal routes in mice. TLR5 can be stimulated in vitro by Flagellin (high Purity) at 5-50ng/mL & in vivo at a starting concentration of 0.1-1ug/mouse.
TRUSTED_SUSTAINABILITY

Specifications

Accession Number P06179
Concentration LYOPH
For Use With (Application) Bioactivity, ELISA
Formulation Lyophilized from PBS
Quantity 10 μg
Storage Requirements Store at 4C short term. Aliquot and Store at -20°C long term. Avoid freeze-thaw cycles.
Endotoxin Concentration <0.01 EU/μg purified protein (LAL test; Lonza).
Reconstitution Reconstitute with 100 ul sterile water (0.1mg/ml after reconstitution)
Research Category Immunology, Innate Immunity
Purity or Quality Grade >95% pure by SDS-PAGE
Protein Flagellin
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