Gibco™ Fetal Bovine Serum, Tet system approved, USDA-approved regions

Product Code.	Quantity	Format	unitSize
16866796	50 mL	One Shot	50mL
16876796	500 mL	Bottle	500mL

Product Code. 16866796 Supplier Gibco™ Supplier No. A4736301

Description

Tet system-approved FBS is formulated for cell culture applications using tetracycline- and tetracycline derivative-regulated gene expression systems.

Tet system-approved FBS is formulated for cell culture applications using tetracycline- and tetracycline derivative-regulated gene expression systems. Cell-based assays that detect the presence of tetracycline and tetracycline analogs are used to select FBS that delivers the highest range of induction.

• Designed to ensure the absence of detectable tetracycline and its derivatives that may interfere with accurate regulation of gene expression
• Functional testing provides confidence that unintended tetracyline gene expression will not result
• Sera category: specialty

Specifications

• Certifications/Compliance: ISO 13485 Certified
• Classification: Value
• Content And Storage: Store at <-10°C.
• Species: Cattle/Bovine
• Cell Type: Use for a broad range of cell types, especially sensitive cell lines
• Age: Fetal
• Country of Origin: Mexico
• Format: One Shot
• Specialty Type: Tet-System Approved
• Product Type: Fetal Bovine Serum
• Quantity: 50 mL
• Sterility: Sterile
• Sterilization Method: Triple-filtered, 0.1 μm
• Grade: Value
• Tested For: 50 quality specification tests including 9 CFR virus testing, endotoxin, and performance
• Endotoxin Concentration: <50 EU/mL (levels routinely <10 EU/mL)
• Green Features: Sustainable packaging
• Hemoglobin Concentration: <25 mg/dL
• Purity or Quality Grade: Value
• Product Line: One Shot
• Shipping Condition: Frozen

Frequently Asked Questions (FAQs)

What are the benefits and disadvantages of using heat-inactivated FBS in cell culture?

1. Heating inactivates complement. Active complement can participate in cytolytic events, contract smooth muscle, release histamine from mast cells and platelets, and activate lymphocytic and macrophage cells. Applications where heat-inactivated serum is recommended include immunological studies and culturing of embryonic stem cells (ESCs), insect cells, and smooth muscle cells.
2. Heat inactivation helps to achieve bottle-to-bottle and lot-to-lot stability by neutralizing many factors that can vary largely from lot to lot.
3. There aren't necessarily disadvantages to heat inactivation of FBS, but there is some evidence that suggests there may be no added benefit to it unless you are carrying out immune studies.

Note: Heat inactivation is performed in a 56 degrees C water bath for 30 min with swirling every 10 min or so for heat distribution and to lower the degree of protein aggregation/flocculant precipitation. Note: If the time or temperature is exceeded, the serum may thicken to a gel. If this occurs, the serum is no longer usable. Unnecessary heat inactivation can take up time and potentially lead to wasted reagents if a mistake is made during the protocol¹.

1. Pellerin, et al., Bioengineering, published in 2021.

Why do you use a cell-based assay instead of HPLC to determine the tetracycline concentration/presence in Fetal Bovine Serum, Tet system approved?

While competitors may use HPLC or other assays to determine the presence of tetracycline, we developed a cell-based assay because we wanted to verify that the tetracycline present, if any, in this product would not be enough to alter the experiment. The only way to confirm this is to actually demonstrate the efficacy of the serum in a dish with cells (cell-based assay).

How is Fetal Bovine Serum, Tet system approved verified to be tetracycline-free?

Fetal Bovine Serum, Tet system approved is not tetracycline-free, and no serum can be absolutely confirmed to be tetracycline-free without altering the performance of the product. We developed a cell-based assay to confirm that tetracycline, if present, is not in high enough concentrations to alter Tet experiments.

Do you offer Fetal Bovine Serum, Tet system approved that has already been heat inactivated?

Sorry, we do not offer Fetal Bovine Serum, Tet system approved that has already been heat inactivated.

Is it normal to have precipitation in Fetal Bovine Serum, Tet system approved?

Since our sera are not pre-aged before filtration, turbidity or flocculent debris may develop upon thawing or storage. This flocculence is a normal characteristic of the serum and does not adversely affect performance of the serum.

Do you have different quality standards for the parent lots of serum that are selected for production of Fetal Bovine Serum, Tet system approved?

No. The parent lots that are assigned to become Fetal Bovine Serum, Tet system approved have no differences in collection or quality standards.

Is Fetal Bovine Serum, Tet system approved sterile?

Fetal Bovine Serum, Tet system approved is triple filtered using a 0.1 µm filter.

For research use or further manufacturing use only. Serum and blood proteins are not for direct administration into humans or animals.