Gibco™ Fetal Bovine Serum, qualified, United States

Product Code.	Quantity	Format	unitSize
15575309	50 mL	One Shot	50mL
15585309	10 x 50 mL	One Shot	Pack of 10
11570636	100 mL	Bottle	100mL

Product Code. 15575309 Supplier Gibco™ Supplier No. A3160501

Description

Important Update: We've recently introduced Premium Fetal Bovine Serum (FBS) specification-based products. These improved products are alternatives to our Qualified US origin SKUs. Product stability, performance, manufacturing, storage, and transport processes are unchanged.

Gibco fetal bovine sera offer excellent value for basic cell culture, specialty research, and specific assays, earning the trust of researchers with consistent quality and award-winning support that helps meet your research needs and budget requirements

Gibco Sera: Trusted Quality for Consistent Performance

Gibco Sera Category: Value Plus (Performance)

• Use for general cell culture with common cell lines
• Low endotoxin and hemoglobin levels
• Endotoxin level: <10 EU/mL
• Hemoglobin level: <25 mg/dL
• Origin: United States

Gibco Serum Delivers

• ISO 13485 certified, processed in FDA registered facilities
• Triple filtered at 0.1 μm
• Gibco bottle is easier to use in the hood, reduces the risk of contamination and helps you perform cell culture more consistently
• Peel-off sticker on label provides handy reference for lot number and expiration date recording in lab notebook

Order Info

Shipping Condition: Dry Ice

Specifications

• Content And Storage: Storage conditions: ≤-10°C
  Shipping conditions: Frozen
• Description: Fetal Bovine Serum, qualified, One Shot™ format, United States
• Species: Bovine
• Age: Fetal
• Country of Origin: United States
• Form: Liquid
• Format: One Shot
• Product Type: Fetal Bovine Serum
• Quantity: 50 mL
• Serum Treatment: Standard (Sterile-filtered)
• Sterility: Sterile
• Sterilization Method: Triple-filtered, 0.1 μm
• Endotoxin Concentration: ≤10 EU/mL
• Hemoglobin Concentration: ≤25 mg/dL
• Purity or Quality Grade: Qualified
• Product Line: One Shot
• Shipping Condition: Frozen

Frequently Asked Questions (FAQs)

What are the benefits and disadvantages of using heat-inactivated FBS in cell culture?

1. Heating inactivates complement. Active complement can participate in cytolytic events, contract smooth muscle, release histamine from mast cells and platelets, and activate lymphocytic and macrophage cells. Applications where heat-inactivated serum is recommended include immunological studies and culturing of embryonic stem cells (ESCs), insect cells, and smooth muscle cells.
2. Heat inactivation helps to achieve bottle-to-bottle and lot-to-lot stability by neutralizing many factors that can vary largely from lot to lot.
3. There aren't necessarily disadvantages to heat inactivation of FBS, but there is some evidence that suggests there may be no added benefit to it unless you are carrying out immune studies.

Note: Heat inactivation is performed in a 56 degrees C water bath for 30 min with swirling every 10 min or so for heat distribution and to lower the degree of protein aggregation/flocculant precipitation. Note: If the time or temperature is exceeded, the serum may thicken to a gel. If this occurs, the serum is no longer usable. Unnecessary heat inactivation can take up time and potentially lead to wasted reagents if a mistake is made during the protocol¹.

1. Pellerin, et al., Bioengineering, published in 2021.

What is the shelf life of Fetal Bovine Serum, qualified, United States once thawed? Can I refreeze it after thawing?

The stability after opening and thawing is application-dependent and most often, it is used within a week or less. The product can be thawed and refrozen. This is standard practice for making aliquots. We recommend that you make smaller aliquots and use as needed.

What is the difference between "qualified" versus "certified" purity grade FBS?

The main difference is in the Quality Control (QC) test specifications. “Qualified” and “Certified” FBS go through the same QC tests but “Certified” FBS has additional QC tests as well as more stringent QC test specifications for endotoxin and hemoglobin levels. The additional QC tests in “Certified” FBS include biochemical and hormonal profiling.

How long can serum be stored at 2-8 degrees C?

Serum can be kept at 2-8 degrees C for up to 4 weeks.

Are there any other packaging configurations available for the new One Shot FBS product?

Yes, we also offer a 10 X 50 mL package, which is equivalent to a 500 mL bottle of FBS.

How is the new One Shot FBS bottle different from our existing One Shot bottle?

Our previous One Shot bottle, a 60 mL Nalgene media bottle, is not designed for easy pipetting and pouring whereas the new One Shot FBS bottle has a propietary design that was developed specifically for FBS use. This design allows you to freeze, thaw and pour without the need to aliquot, which minimizes the amount of FBS handling and reduces the risk of contamination.

Does the new One Shot FBS bottle fit into a conical tube rack?

The new One Shot FBS bottle is not shaped to fit into a traditional conical tube rack. It does however fit nicely in a 30 mm test tube rack for the purposes of storage and use in a water bath and biological safety cabinet.

What is the best way to hold the new One Shot FBS bottle for pouring?

For optimal, burp-free pouring, securely grip the bottle using the indents and pour with the chamfered edge facing down.

What is the shelf life of the new One Shot FBS?

The initial shelf life/stability for the new One Shot FBS is two years from the date of manufacture. Additional updates will continue to be made as data becomes available.

Can I heat inactivate the serum in the new One Shot FBS bottle?

Yes, the serum in the new One Shot FBS bottle can be heat inactivated at 56 degrees C for 30 minutes.

Can I reuse the new One Shot FBS bottles?

It is at the discretion of the customer to reuse these bottles for purposes other than what they are intended for. But please note that they cannot be autoclaved.

What type of plastic is the new One Shot FBS bottle made from?

It is made from polyethylene terephthalate (PET, PETE). This is the same plastic used in our other bottles.

For research use or further manufacturing use only. Serum and blood proteins are not for direct administration into humans or animals.