Invitrogen™ Ferritin Human ELISA Kit

Sandwich ELISA Kit

Product Code	Brand	Quantity
15450333	Invitrogen™ EHFTL	96 Tests
17155449	Invitrogen™ EHFTLX5	5 x 96 Tests

Description

The Human Ferritin ELISA quantitates Hu Ferritin in human serum, plasma, or cell culture medium. The assay will exclusively recognize both natural and recombinant Hu Ferritin. Principle of the method The Human Ferritin solid-phase sandwich ELISA (enzyme-linked immunosorbent assay) is designed to measure the amount of the target bound between a matched antibody pair. A target-specific antibody has been pre-coated in the wells of the supplied microplate. Samples, standards, or controls are then added into these wells and bind to the immobilized (capture) antibody. The sandwich is formed by the addition of the second (detector) antibody, a substrate solution is added that reacts with the enzyme-antibody-target complex to produce measurable signal. The intensity of this signal is directly proportional to the concentration of target present in the original specimen. Rigorous validation Each manufactured lot of this ELISA kit is quality tested for criteria such as sensitivity, specificity, precision, and lot-to-lot consistency. See manual for more information on validation.

Ferritin is the major intracellular iron storage protein in prokaryotes and eukaryotes. Ferritin is composed of 24 subunits of the heavy and light ferritin chains. Variation in ferritin subunit composition may affect the rates of iron uptake and release in different tissues. A major function of ferritin is the storage of iron in a soluble and nontoxic state. Defects in the light chain ferritin gene are associated with several neurodegenerative diseases and hyperferritinemia-cataract syndrome. The ferritin gene has multiple pseudogenes, and ferritin is a ubiquitous and highly conserved protein which plays a major role in iron homeostasis. Ferritin is a holoenzyme shell with a molecular weight of about 450 kDa, and consists of 24 subunits of two types, H(heavy) and L(light), and capable of storing up to 4,500 atoms of ferric iron.

Specifications

• Accession Number: P02792,P02794
• Description: Ferritin ELISA Kit, 96 Assays, 1 Kit, Human Species, Other Proteins Protein Family, Inflammation Research Discipline, Enzyme-Linked Immunosorbent Assay Designed for the Quantitation and Detection of Ferritin in Samples Using a Microplate Reader
• Assay: Solid-phase sandwich ELISA
• Detection Method: Colorimetric
• Protein Family: Other Proteins
• Specificity: This Ferritin ELISA kit shows no cross-reactivity with the following cytokines tested: human Angiogenin, BDNF, BLC, CNTF, ENA- 78, FGF-4, IL-1 alpha, IL-1 beta, IL-2, IL-3, IL-4, IL-5, IL-6, IL-7, IL-8, IL-9, IL- 11, IL-12 p70, IL-12 p40, IL-13, IL-15, IL-309, IP-10, FGF-4, FGF-6, FGF- 7, G-CSF, GDNF, GM-CSF, IFN-gamma, IGFBP-2, IGFBP-3, IGFBP-4, Leptin (OB), MCP-1, MCP-2, MCP-3, MDC, MIF, MIG, MIP-1 alpha, MIP-1 beta, MIP-1 delta, PARC, PDGF, RANTES, SCF, SDF-1 alpha, TARC, TGF-beta, TIMP-1, TIMP-2, TNF-alpha, TNF-beta, TPO, VEGF.
• Target Species: Human
• For Use With (Equipment): Colorimetric Microplate Reader
• Gene Symbol: FHC,FTH,FTHL6,HFE5,PIG15,PLIF,LFTD,NBIA3
• Interassay CV: <12%
• Label or Dye: HRP
• Research Discipline: Inflammation
• Synonym: LFTD, NBIA3
• Test Time: 1 hr. 20 min.

• Assay Sensitivity: 600 pg/mL
• Detectable Analytes: Ferritin
• Conjugate: Biotin
• Product Type: ELISA Kit
• Sample Type: Plasma, Serum, Supernatant
• Substrate Type: TMB
• For Use With (Application): ELISA, Protein Assays, Protein Biology, Cancer Biology, Immunology, Neuroscience, Neurobiology, Protein Detection, Protein Analysis
• Gene ID (Entrez): 2495, 2512
• Host Species: Human
• Intraassay CV: <10%
• Regulatory Status: RUO
• Storage Requirements: 2°C to 8°C
• Target: Human
• Total Assay Time: 4 hr. 45 min.