Thermo Scientific™ VspI (AseI)

Cut at AT^TAAT sites with VspI (AseI) restriction enzyme, which performs best at 37°C in O buffer (Isoschizomers: AseI, PshBI).

Overview
Brand: Thermo Scientific™

Manufacturer Part Number: ER0911

VSPI (ASEI) 10U/UL 1000U

UNSPSC: 12352204

Code: 88

Additional Details:
Additional Details: Weight: 1.51950kg



Product Code. 10329869

Quantity Price
1 £ 24.97 / 1000 units
EU Stock 19
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Description and Specification

Specification

Product Name VspI (AseI)
Concentration 10U/µL
Units 1,000U

5'..A T^T AA T...3'

3'..T AA T^T A...5'

Conditions for 100% Activity

  • 1X Buffer O:50mM Tris-HCl (pH 7.5 at 37°C), 10 mM MgCl2, 100 mM NaCl and 0.1mg/ml BSA
  • Incubate at 37°C

Storage Buffer

  • VspI is supplied in: 10mM Tris-HCl (pH 7.4 at 25°C), 100mM KCl, 1mM DTT, 1mM EDTA, 0.2mg/ml BSA and 50% (v/v) glycerol

Ligation and Recleavage

  • After 50-fold overdigestion with VspI, more than 95% of DNA fragments can be ligated and recut

Methylation Effects

  • Dam: never overlaps – no effect
  • Dcm: never overlaps – no effect
  • CpG: never overlaps – no effect
  • EcoKI: never overlaps – no effect
  • EcoBI: may overlap – effect not determined

Digestion of Agarose-embedded DNA

  • Minimum 5 units of the enzyme are required for complete digestion of 1μg of agarose-embedded lambda DNA in 16 hours

Compatible Ends

  • Csp6I, FspBI, NdeI, Tru1I