Thermo Scientific™ TrueStart Hot Start Taq DNA Polymerase

Improve specificity and sensitivity of hot-start PCR with this polymerase.

Brand:  Thermo Scientific™ EP0612

Product Code. 10540081

Description

TrueStart Hot Start Taq DNA Polymerase is designed for hot start PCR, a technique shown to improve specificity, sensitivity and yield of DNA amplification during PCR. TrueStart Hot Start Taq DNA Polymerase is chemically modified by adding proprietary heat-labile blocking groups to amino acid residues. TrueStart Hot Start Taq DNA Polymerase is inactive at room temperature because of this modification, avoiding extension of non-specifically annealed primers or primer dimers.

TrueStart Hot Start Taq DNA Polymerase activates rapidly during the initial denaturation step of PCR. The lack of additional activation step differentiates it from other hot start DNA polymerases, making this enzyme an ideal tool for both automatic hot start PCR and fast PCR. Activated TrueStart Hot Start Taq DNA Polymerase catalyzes 5'-3' synthesis of DNA, lacks detectable 3'-5' exonuclease (proofreading) activity, but possesses low 5'-3' exonuclease activity. These two activities are not detectable before activation.

Highlights

Fastest activating hot start Taq DNA polymerase

High PCR specificity -reduced effects of mis-priming and primer-dimer formation

Enhanced PCR sensitivity

Convenient room temperature PCR set-up

Generates PCR products with 3'-dA overhangs

Specifications

• Description: TrueStart Hot Start Taq DNA Polymerase
• Quantity: 500U
• For Use With (Application): Standard PCR, RT-PCR, Real Time PCR (qPCR)
• Includes: 2 x 1.25mL 10X TrueStart Taq Buffer and 2 x 1.25mL 25mM MgCl2

In certain countries use of this product is covered by patents. Purchase of product in these countries includes non-transferable, limited license for using only this amount of product for the purchaser's own internal research.

This product or the use of this product is covered by US patent application US20070224611A1 and corresponding counterparts owned by Fermentas. The purchase of this product includes a non-transferable license to use this product for the purchaser's internal research. All other commercial uses of this product, including without limitation product use for diagnostic purposes, resale of product in the original or any modified form or product use in providing commercial services require a separate license from Fermentas.