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Thermo Scientific™ Taq DNA Polymerase, recombinant (5 U/μL)

Thermo Scientific Taq DNA Polymerase is a highly thermostable DNA polymerase from the thermophilic bacterium Thermus aquaticus.

£44.77 - £1330.00

Specifications

GC-Rich PCR Performance Low
Polymerase Taq DNA Polymerase
Reaction Speed Standard
Product Type Taq DNA Polymerase (Recombinant)
Shipping Condition Dry Ice
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Products 4
Product Code Brand Quantity Price Quantity & Availability  
Product Code Brand Quantity Price Quantity & Availability  
10374980
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Thermo Scientific™
EP0401
100 Units
£44.77
100 units
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10733941
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Thermo Scientific™
EP0402
500 Units
£179.00
500 units
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10349509
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Thermo Scientific™
EP0405
5 x 500 Units
£802.00
Pack of 5
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10272580
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Thermo Scientific™
EP0406
10 x 500 Units
£1330.00
Pack of 10
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Description

Description

Thermo Scientific Taq DNA Polymerase is a highly thermostable DNA polymerase from the thermophilic bacterium Thermus aquaticus. The enzyme catalyzes 5' to 3' synthesis of DNA, has no detectable 3' to 5' exonuclease (proofreading) activity, and possesses low 5' to 3' exonuclease activity. In addition, Taq DNA Polymerase exhibits deoxynucleotidyl transferase activity, which frequently results in the addition of extra adenines at the 3'-end of PCR products. Recombinant Taq DNA Polymerase is the ideal tool for standard PCR of templates 5 kb or shorter.

Features

  • Thermostable—half life is more than 40 min at 95°C
  • Generates PCR products with 3'-dA overhangs
  • Supplied with two buffers: 10X Taq Buffer with KCl and 10X Taq Buffer with (NH4)2SO4
  • Incorporates modified nucleotides (e.g., biotin-, digoxigenin-, fluorescent-labeled nucleotides)

Applications

  • Routine PCR amplification of DNA fragments up to 5 kb
  • High throughput PCR
  • DNA labeling

Notes

  • The error rate of Taq DNA Polymerase in PCR is 2.2 x 10-5 errors per nt per cycle. Accordingly, the accuracy of PCR is 4.5 x 104. Accuracy is an inverse of the error rate and shows an average number of correct nucleotides incorporated before an error occurs.
  • 10X Taq Buffer without Detergent is recommended for microarray experiments.
  • 10X Taq Buffer with (NH4)2SO4 allows for PCR at wide range of magnesium concentrations and decreases unspecific priming.
Specifications

Specifications

Low
Standard
Dry Ice
5 U/μL
No
Separate Components
Taq DNA Polymerase
Taq DNA Polymerase (Recombinant)
Standard PCR
1X
3'-A
Videos
SDS
Documents

Documents

Product Certifications

For Research Use Only. Not for use in diagnostic procedures.