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Thermo Scientific™ TaaI (HpyCH4III)

The TaaI (HpyCH4III) restriction enzyme recognizes ACN^GT sites and cuts best at 65°C in Tango buffer (Isoschizomers: Bst4CI, HpyCH4III).

Brand:  Thermo Scientific™ ER1362

Code : 88

Additional Details : Weight : 0.00900kg

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Product Code. 10723911

  • £148.00 / 1000 units


EU Stock 3
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Description

Description

5'...A C N▵G T...3'

3'...T G▵N C A...5'

Conditions for 100% Activity

  • 1X Buffer Tango: 33mM Tris-acetate (pH7.9 at 37°C), 10mM Mg-acetate, 66mM K-acetate and 0.1mg/mL BSA
  • Incubate at 65°C
  • To ensure higher efficiency of digestion, perform the cleavage reaction under paraffin oil

Storage Buffer

  • TaaI is supplied in:10mM Tris-HCl (pH7.5 at 25°C), 100mM KCl, 1mM DTT, 0.1mM EDTA, 0.2mg/mL BSA and 50% (v/v) glycerol

Ligation and Recleavage

  • After 50-fold overdigestion with TaaI, approximately 90% of the DNA fragments can be ligated and recut

Methylation Effects

  • Dam: never overlaps — no effect
  • Dcm: never overlaps — no effect
  • CpG: may overlap — cleavage impaired
  • EcoKI: may overlap — effect not determined
  • EcoBI: may overlap — effect not determined

Compatible Ends

  • Hpy188I

Note:

Incubation at 37°C results in 10% activity.

Specifications

Specifications

TaaI (HpyCH4III)
10U/μL
1000U
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