Thermo Scientific™ T7 RNA Polymerase

Catalyze the 5'=>3' synthesis of RNA on either single-stranded or double-stranded DNA with T7 RNA polymerase.

Overview
Brand: Thermo Scientific™

Manufacturer Part Number: EP0112

MODIFYING ENZYME, T7 RNA POLYMERASE, 20U/UL,(supplied with 5 x 1mL of 5X transcription buffer)

UNSPSC: 41105518

Code: 88

Additional Details:
Additional Details: Weight: 1.51950kg



Product Code. 10212610

Quantity Price
1 £ 109.0 / 25000 units
EU Stock 9
Add to basket


Description and Specification

Specification

Concentration 20U/┬ÁL
Description T7 RNA Polymerase
For Use With (Application) Synthesis of unlabeled and labeled RNA that can be used:for hybridization (1), in vitro RNA translation (2); as aRNA (3), siRNA (4), substrate in RNase protection assays (5), template for genomic DNA sequencing.
Includes 5 x 1mL of 5X Transcription Buffer
Quantity 5 x 5000U

It catalyzes the 5'=>3' synthesis of RNA on either single-stranded DNA or double-stranded DNA downstream from it promoter.
  • Incorporates modified nucleotides (e.g., aminoallyl-, biotin-, fluorescein-, digoxigenin-labeled nucleotides)

Recommended for:

Synthesis of unlabeled and labeled RNA that can be used:for hybridization, in vitro RNA translation; as aRNA, siRNA, substrate in RNase protection assays, template for genomic DNA sequencing.

Note:

Consensus promoter sequences: TAATACGACTCACTATA G GGAGA. The position in bold (+1) indicates the first nucleotide incorporated into RNA during transcription. Only bases at this position through +3 are critical for transcription, and they must be a G and a purine base, respectively.