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Thermo Scientific™ T3 RNA Polymerase

Catalyze the 5'→3' synthesis of RNA on either single-stranded or double-stranded DNA with T73 RNA polymerase.

Brand:  Thermo Scientific™ EP0102

UNSPSC : 41105518

Code : 88

Additional Details : Weight : 0.01000kg

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Product Code. 10849020

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Description

Description

It catalyzes the 5'=>3' synthesis of RNA on either single-stranded DNA or double-stranded DNA downstream from its promoter.
  • Incorporates modified nucleotides (e.g., aminoallyl-, biotin-, fluorescein-, digoxigenin-labeled nucleotides)

Recommended for:

Synthesis of unlabeled and labeled RNA that can be used: for hybridization (1), in vitro RNA translation (2); as aRNA (3), siRNA (4), substrate in RNase protection assays (5), template for genomic DNA sequencing.

Note:

Consensus promoter sequences: AATTAACCCTCACTAAA G GGAGA–T3 TAATACGACTCACTATA G GGAGA–T7 ATTTAGGTGACACTATA G AAGNG–SP6 The position in bold (+1) indicates the first nucleotide incorporated into RNA during transcription. Only bases at this position through +3 are critical for transcription, and they must be a G and a purine base, respectively (9).

Specifications

Specifications

20U/µL
Synthesis of unlabeled and labeled RNA that can be used: for hybridization (1), in vitro RNA translation (2); as aRNA (3), siRNA (4), substrate in RNase protection assays (5), template for genomic DNA sequencing.
10,000U
T3 RNA Polymerase
1mL of 5X Transcription Buffer
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