Thermo Scientific™ T3 RNA Polymerase

Catalyze the 5'→3' synthesis of RNA on either single-stranded or double-stranded DNA with T73 RNA polymerase.

Overview
Brand: Thermo Scientific™

Manufacturer Part Number: EP0103

MODIFYING ENZYME, T3 RNA POLYMERASE, >100U/UL,HC, (supplied with 2x1mL of 5X transcription

UNSPSC: 41105518

Code: 88

Additional Details:
Additional Details: Weight: 0.01000kg



Product Code. 10680101

Quantity Price
1 £ 84.25 / 10000 units
Estimated Shipment date
from Supplier 17-03-2017
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Description and Specification

Specification

Concentration 200U/µL
Quantity 10,000U
Description T3 RNA Polymerase
For Use With (Application) Synthesis of unlabeled and labeled RNA that can be used: for hybridization (1), in vitro RNA translation (2); as aRNA (3), siRNA (4), substrate in RNase protection assays (5), template for genomic DNA sequencing.
Includes 1mL of 5X Transcription Buffer
Quantity 10,000U
Item Description HC, 10000U

It catalyzes the 5'=>3' synthesis of RNA on either single-stranded DNA or double-stranded DNA downstream from its promoter.
  • Incorporates modified nucleotides (e.g., aminoallyl-, biotin-, fluorescein-, digoxigenin-labeled nucleotides)

Recommended for:

Synthesis of unlabeled and labeled RNA that can be used: for hybridization (1), in vitro RNA translation (2); as aRNA (3), siRNA (4), substrate in RNase protection assays (5), template for genomic DNA sequencing.

Note:

Consensus promoter sequences: AATTAACCCTCACTAAA G GGAGA–T3 TAATACGACTCACTATA G GGAGA–T7 ATTTAGGTGACACTATA G AAGNG–SP6 The position in bold (+1) indicates the first nucleotide incorporated into RNA during transcription. Only bases at this position through +3 are critical for transcription, and they must be a G and a purine base, respectively (9).