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Thermo Scientific™ SspI

The SspI restriction enzyme recognizes AAT^ATT sites and cuts best at 37°C in G buffer.

Brand:  Thermo Scientific™ ER0772

Code : 88

Additional Details : Weight : 0.01000kg

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Product Code. 10203120

  • £118.00 / 2500 units


EU Stock 2
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Description

Description

5'...A A T▵A T T...3'

3'...T T A▵T A A...5'

Conditions for 100% Activity

  • 1X Buffer G:10mM Tris-HCl (pH7.5 at 37°C), 10mM MgCl2, 50mM NaCl and 0.1mg/mL BSA.Incubate at 37°C

Storage Buffer

  • SspI is supplied in:10mM Tris-HCl (pH7.4 at 25°C), 100mM KCl, 1mM DTT, 1mM EDTA, 0.2mg/mL BSA and 50% (v/v) glycerol

Ligation and Recleavage

  • After 10-fold overdigestion with SspI, more than 90% of the DNA fragments can be ligated and recut

Methylation Effects

  • Dam: never overlaps — no effect
  • Dcm: never overlaps — no effect
  • CpG: never overlaps — no effect
  • EcoKI: never overlaps — no effect
  • EcoBI: may overlap — no effect

Digestion of Agarose-embedded DNA

  • Minimum 5units of the enzyme are required for complete digestion of 1μg of agarose-embedded lambda DNA in 16hours

Note:

Greater than 10-fold overdigestion with SspI may result in star activity.

Specifications

Specifications

SspI
10U/┬ÁL
2,500U