Thermo Scientific™ SP6 RNA Polymerase

Catalyze the 5'→3' synthesis of RNA on either single-stranded or double-stranded DNA with Thermo Scientific™ SP6 RNA polymerase.

Overview
Brand: Thermo Scientific™

Manufacturer Part Number: EP0133

MODIFYING ENZYME, SP6 RNA POLYMERASE, >100U/UL,(supplied with 1mL of 5X transcription buffer)

UNSPSC: 12352204

Code: 88

Additional Details:
Additional Details: Weight: 0.00900kg



Product Code. 10899720

Quantity Price
1 £ 129.0 / 5000 units
EU Stock 7
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Description and Specification

Specification

Description SP6 RNA Polymerase
For Use With (Application) Synthesis of unlabeled and labeled RNA that can be used: for hybridization (1), in vitro RNA translation (2); as aRNA (3), siRNA (4), substrate in RNase protection assays (5), template for genomic DNA sequencing.
Includes 1mL of 5X Transcription Buffer
Quantity 1000U

It catalyzes the 5'=>3' synthesis of RNA on either single-stranded DNA or double-stranded DNA downstream from it promoter.
  • Incorporates modified nucleotides (e.g., aminoallyl-, biotin-, fluorescein-, digoxigenin-labeled nucleotides)

Recommended for:

Synthesis of unlabeled and labeled RNA that can be used: for hybridization (1), in vitro RNA translation (2); as aRNA (3), siRNA (4), substrate in RNase protection assays (5), template for genomic DNA sequencing.

Note:

Consensus promoter sequences: AATTAACCCTCACTAAA G GGAGA - T3 TAATACGACTCACTATA G GGAGA - T7 ATTTAGGTGACACTATA G AAGNG - SP6 The position in bold (+1) indicates the first nucleotide incorporated into RNA during transcription. Only bases at this position through +3 are critical for transcription, and they must be a G and a purine base, respectively (9).