Thermo Scientific™ SalI

The SalI restriction enzyme recognizes G^TCGAC sites and cuts best at 37°C in O buffer.

Overview
Brand: Thermo Scientific™

Manufacturer Part Number: ER0642

SALI 10U/UL 5X1500U

UNSPSC: 12352204

Code: 88

Additional Details:
Additional Details: Weight: 0.02000kg



Product Code. 10242440

Quantity Price
1 £ 138.0 / Pack of 5
EU Stock 13
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Description and Specification

Specification

Product Name SalI
Concentration 10U/┬ÁL
Units 5 x 1,500U

5'...G▵T C G A C...3'

3'...C A G C T▵G...5'

Conditions for 100% Activity

  • 1X Buffer O:50mM Tris-HCl (pH7.5 at 37°C), 10mM MgCl2, 100mM NaCl and 0.1mg/mL BSA
  • Incubate at 37°C

Storage Buffer

  • SalI is supplied in:10mM Tris-HCl (pH7.4 at 25°C),100mM KCl, 1mM DTT, 0.1mM EDTA, 0.2mg/mL BSA and 50% (v/v) glycerol

Ligation and Recleavage

  • After 50-fold overdigestion with SalI, more than 95% of the DNA fragments can be ligated and recut

Methylation Effects

  • Dam: never overlaps — no effect
  • Dcm: never overlaps — no effect
  • CpG: completely overlaps — blocked
  • EcoKI: never overlaps — no effect
  • EcoBI: never overlaps — no effect

Digestion of Agarose-embedded DNA

  • Minimum 5units of the enzyme are required for complete digestion of 1μg of agarose-embedded lambda DNA in 16hours

Compatible Ends

  • Eco88I, SmoI, XhoI

Note:

Low salt, high glycerol (>5%) concentrations, pH >8.0 or a large excess of enzyme may result in star activity. Supercoiled forms of pBR322 and pUC require 10-fold overdigestion with SalI to achieve complete digestion. Incubation at 25°C results in 50-75% activity.