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Thermo Scientific™ SalI

The SalI restriction enzyme recognizes G^TCGAC sites and cuts best at 37°C in O buffer.

Brand:  Thermo Scientific™ ER0642

Code : 88

Additional Details : Weight : 0.02000kg

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Product Code. 10242440

  • £138.00 / Pack of 5


EU Stock 8
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Description

Description

5'...G▵T C G A C...3'

3'...C A G C T▵G...5'

Conditions for 100% Activity

  • 1X Buffer O:50mM Tris-HCl (pH7.5 at 37°C), 10mM MgCl2, 100mM NaCl and 0.1mg/mL BSA
  • Incubate at 37°C

Storage Buffer

  • SalI is supplied in:10mM Tris-HCl (pH7.4 at 25°C),100mM KCl, 1mM DTT, 0.1mM EDTA, 0.2mg/mL BSA and 50% (v/v) glycerol

Ligation and Recleavage

  • After 50-fold overdigestion with SalI, more than 95% of the DNA fragments can be ligated and recut

Methylation Effects

  • Dam: never overlaps — no effect
  • Dcm: never overlaps — no effect
  • CpG: completely overlaps — blocked
  • EcoKI: never overlaps — no effect
  • EcoBI: never overlaps — no effect

Digestion of Agarose-embedded DNA

  • Minimum 5units of the enzyme are required for complete digestion of 1μg of agarose-embedded lambda DNA in 16hours

Compatible Ends

  • Eco88I, SmoI, XhoI

Note:

Low salt, high glycerol (>5%) concentrations, pH >8.0 or a large excess of enzyme may result in star activity. Supercoiled forms of pBR322 and pUC require 10-fold overdigestion with SalI to achieve complete digestion. Incubation at 25°C results in 50-75% activity.

Specifications

Specifications

SalI
10U/μL
5 x 1,500U
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