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Thermo Scientific™ RsaI

The RsaI restriction enzyme recognizes GT^AC sites and cuts best at 37°C in Tango buffer (Isoschizomers: AfaI, CviQI, RsaNI).

Brand:  Thermo Scientific™ ER1122

UNSPSC : 12352204

Code : 88

Additional Details : Weight : 0.01000kg

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Product Code. 10359509

  • £181.00 / 1000 units

EU Stock 5
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5'...G T▵A C...3'

3'...C A▵T G...5'

Conditions for 100% Activity

  • 1X Buffer Tango: 33mM Tris-acetate (pH 7.9 at 37°C), 10mM Mg-acetate, 66mM K-acetate and 0.1mg/mL BSA
  • Incubate at 37°C

Storage Buffer

  • RsaI is supplied in:10mM Tris-HCl (pH 7.4 at 25°C), 100mM KCl, 1mM DTT, 1mM EDTA, 0.2mg/mL BSA and 50% (v/v) glycerol

Ligation and Recleavage

  • After 50-fold overdigestion with RsaI, more than 95% of the DNA fragments can be ligated and recut

Methylation Effect

  • Dam: never overlaps —no effect
  • Dcm: never overlaps —no effect
  • CpG: may overlap —cleavage impaired
  • EcoKI: never overlaps —no effect
  • EcoBI: never overlaps —no effect