Thermo Scientific™ RsaI

The RsaI restriction enzyme recognizes GT^AC sites and cuts best at 37°C in Tango buffer (Isoschizomers: AfaI, CviQI, RsaNI).

Overview
Brand: Thermo Scientific™

Manufacturer Part Number: ER1121

1000 UNIT RESTRICTION ENZYME, CONVENTIONAL, RSAI,10u/ul, cuts GT¬AC at 37°C (supplied with 1mL of

UNSPSC: 12352204

Code: 88

Additional Details:
Additional Details: Weight: 1.51950kg



Product Code. 10334380

Quantity Price
1 £ 45.88 / Each
EU Stock 34
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Description and Specification

Specification

Product Name RsaI
Concentration 10U/µL
Units 1,000U

5'...G T▵A C...3'

3'...C A▵T G...5'

Conditions for 100% Activity

  • 1X Buffer Tango: 33mM Tris-acetate (pH 7.9 at 37°C), 10mM Mg-acetate, 66mM K-acetate and 0.1mg/mL BSA
  • Incubate at 37°C

Storage Buffer

  • RsaI is supplied in:10mM Tris-HCl (pH 7.4 at 25°C), 100mM KCl, 1mM DTT, 1mM EDTA, 0.2mg/mL BSA and 50% (v/v) glycerol

Ligation and Recleavage

  • After 50-fold overdigestion with RsaI, more than 95% of the DNA fragments can be ligated and recut

Methylation Effect

  • Dam: never overlaps —no effect
  • Dcm: never overlaps —no effect
  • CpG: may overlap —cleavage impaired
  • EcoKI: never overlaps —no effect
  • EcoBI: never overlaps —no effect