Thermo Scientific™ RNase T1 (1000 U/μL)

Product Code.	Quantity	Unit Size
10621621	500,000 units	500000 units
10314820	100,000 units	100000 units

Product Code. 10621621 Supplier Thermo Scientific™ Supplier No. EN0542

Descripción

RNase T1 is an endoribonuclease that specifically degrades single-stranded RNA at G residues.

Thermo Scientific Rnase T1 is an endoribonuclease that specifically degrades single-stranded RNA at G residues (see Figure 1 in Supporting Data). It cleaves the phosphodiester bond between the 3'-guanylic residue and the 5'-OH residue of adjacent nucleotides with the formation of corresponding intermediate 2', 3'-cyclic phosphate. The reaction products are 3'-GMP and oligonucleotides with a terminal 3'-GMP.

Rnase T1 does not require metal ions for activity.

Applications

• Removal of RNA from DNA preparations
• RNA sequencing
• Ribonuclease protection assays. Used in conjunction with RNase A
• Removal of RNA from recombinant protein preparations
• Determination of the level of RNA transcripts synthesized in vitro from DNA templates containing a "G-less cassette"

Especificaciones

• Concentration: 1000 U/μL
• Enzyme: RNase
• Quantity: 500,000 units
• Product Type: RNase T1

Frequently Asked Questions (FAQs)

How much Thermo Scientific RNase T1 should I use for complete digestion of RNA?

We would recommend 0.2 µL RNase T1 per µg of RNA in 50 mM Tris-HCl (pH 7.5) and 1 mM EDTA. Incubate at 37 degrees C for 30 min.

For Research Use Only. Not for use in diagnostic procedures.