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Thermo Scientific™ RevertAid Reverse Transcriptase (200 U/µL)

Perform efficient synthesis of full-length first strand cDNA up to 13 kb with this genetically modified M-MuLV RT for routine reverse transcription.

Brand:  Thermo Scientific™ EP0442

209.70 GBP valid until 2022-06-30
Use promo code "16496" to get your promotional price.



Code : 88

Additional Details : Weight : 0.01260kg

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Product Code. 10161310

  • £245.00 / Pack of 5
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Description

Description

Thermo Scientific RevertAid Reverse Transcriptase (RT) is a recombinant M-MuLV RT. It differs from the M-MuLV RT by its structure and catalytic properties. The enzyme possesses an RNA-dependent and DNA-dependent polymerase activity and a RNase H activity specific to RNA in RNA-DNA hybrids which is significantly lower than that of Avian Myeloblastosis Virus (AMV) reverse transcriptase.

The enzyme possesses an RNA-dependent and DNA-dependent polymerase activity and a RNase H activity specific to RNA in RNA-DNA hybrids which is significantly lower than that of Avian Myeloblastosis Virus (AMV) reverse transcriptase (1, 2).
  • Efficient synthesis of full-length first strand cDNA up to 13kb
  • Optimum activity at 42°C
  • Active up to 50°C
  • Incorporates modified nucleotides (e.g., Cy3-, Cy5-, rhodamine-, aminoallyl-, fluorescein-labeled nucleotides)

Recommended for:

First strand cDNA synthesis for RT-PCR and real-time RT-PCR; Synthesis of cDNA for cloning and expression; Generation of labeled cDNA probes for microarrays; DNA labeling (3)

Specifications

Specifications

42° C
RevertAid
5 x 10000 unit
5 x 1mL of 5X Reaction Buffer
RevertAid
Yes
13 kb or less
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