Thermo Scientific™ PvuII

The PvuII restriction enzyme recognizes CAG^CTG sites and cuts best at 37°C in G buffer.

Overview
Brand: Thermo Scientific™

Manufacturer Part Number: ER0633

RESTRICTION ENZYME, CONVENTIONAL, PVUII, 50U/UL,HC, cuts CAG¬CTG at 37°C (supplied with 1mL of

UNSPSC: 12352204

Code: 88

Additional Details:
Additional Details: Weight: 0.01000kg



Product Code. 10222680

Quantity Price
1 £ 92.25 / 12500 units
EU Stock 1
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Description and Specification

Specification

Product Name PvuII
Concentration 50U/µL
Units 12,500U

5'...C A G▵C T G...3'

3'...G T C▵G A C...5'

Conditions for 100% Activity

  • 1X Buffer G:10mM Tris-HCl (pH 7.5 at 37°C), 10mM MgCl2, 50mM NaCl and 0.1mg/mL BSA
  • Incubate at 37°C

Storage Buffer

  • PvuII is supplied in:10mM Tris-HCl (pH 7.4 at 25°C), 100mM KCl, 1mM DTT, 1mM EDTA, 0.2mg/mL BSA and 50% (v/v) glycerol

Ligation and Recleavage

  • After 10-fold overdigestion with PvuII, more than 90% of the DNA fragments can be ligated and more than 95% of these can be recut

Methylation Effects

  • Dam: never overlaps —no effect
  • Dcm: never overlaps —no effect
  • CpG: never overlaps —no effect
  • EcoKI: never overlaps —no effect
  • EcoBI: may overlap —effect not determined

Digestion of Agarose-embedded DNA

  • Minimum 5units of the enzyme are required for complete digestion of 1μg of agarose-embedded lambda DNA in 16hours

Note:

Greater than 15-fold overdigestion with PvuII may result in star activity.