Thermo Scientific™ PvuII, HC (50 U/μL)

Product Code.	Quantity	unitSize
10222680	12,500 units	12500 units

Product Code. 10222680 Supplier Thermo Scientific™ Supplier No. ER0633

Description

Thermo Scientific™ Fermentas PvuII is available as a FastDigest enzyme which is used for rapid DNA digestion.

Thermo Scientific™ PvuII is available as a FastDigest enzyme which is used for rapid DNA digestion, more than 90% of the digested DNA fragments can be ligated and more than 95% of these sites can be recut at a 5'-termini concentration of 0.15 μM after a 10-fold overdigestion (5U/μg DNA x 2 hours) with PvuII.

5'...C A G▵C T G...3'

3'...G T C▵G A C...5'

Conditions for 100% Activity

• 1X Buffer G:10mM Tris-HCl (pH 7.5 at 37°C), 10mM MgCl₂, 50mM NaCl and 0.1mg/mL BSA
• Incubate at 37°C

Storage Buffer

• PvuII is supplied in:10mM Tris-HCl (pH 7.4 at 25°C), 100mM KCl, 1mM DTT, 1mM EDTA, 0.2mg/mL BSA and 50% (v/v) glycerol

Ligation and Recleavage

• After 10-fold overdigestion with PvuII, more than 90% of the DNA fragments can be ligated and more than 95% of these can be recut

Methylation Effects

• Dam: never overlaps —no effect
• Dcm: never overlaps —no effect
• CpG: never overlaps —no effect
• EcoKI: never overlaps —no effect
• EcoBI: may overlap —effect not determined

Digestion of Agarose-embedded DNA

• Minimum 5units of the enzyme are required for complete digestion of 1μg of agarose-embedded lambda DNA in 16hours

Note:

Greater than 15-fold overdigestion with PvuII may result in star activity.

Specifications

• Concentration: 50 U/μL
• Methylation Sensitivity: Not Dam Methylation-Sensitive, Not Dcm Methylation-Sensitive, Not CpG Methylation-Sensitive
• Enzyme: Pvu II
• Compatible Buffer: 10X Buffer G
• Sensitive to Heat Inactivation: No
• Optimal Reaction Temperature: 37°C
• Type IIS RE: No
• Quantity: 12,500 units
• Product Type: Restriction Enzyme
• Research Category: Traditional Cloning

For Research Use Only. Not for use in diagnostic procedures.