Thermo Scientific™ PvuI

The PvuI restriction enzyme recognizes CGAT^CG sites and cuts best at 37°C in R buffer (Isoschizomers: BpvUI, MvrI, Ple19I).

Brand: Thermo Scientific™

Manufacturer Part Number: ER0622

RESTRICTION ENZYME, CONVENTIONAL, PVUI, 10U/UL,cuts CGAT¬CG at 37°C (suppliedwith 1mL of 10X

UNSPSC: 12352204

Code: 88

Additional Details:
Additional Details: Weight: 0.01000kg

Product Code. 10141220

Quantity Price
1 £234.00 / 1500 units
EU Stock 14
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Description and Specification


Product Name PvuI
Concentration 10U/µL
Units 1,500U

5'..C G A T▵C G...3'

3'..G C▵T A G C...5'

Conditions for 100% Activity

  • 1X Buffer R:10mM Tris-HCl (pH 8.5 at 37°C), 10mM MgCl2, 100mM KCl and 0.1mg/mL BSA
  • Incubate at 37°C

Storage Buffer

  • PvuI is supplied in:10mM Tris-HCl (pH 7.5 at 25°C), 300mM KCl, 0.1mM EDTA, 1mM DTT, 0.2mg/mL BSA and 50% (v/v) glycerol

Ligation and Recleavage

  • After 50-fold overdigestion with PvuI, more than 90% of the DNA fragments can be ligated and more than 95% of these can be recut

Methylation Effects

  • Dam: completely overlaps —no effect
  • Dcm: never overlaps —no effect
  • CpG: completely overlaps —blocked
  • EcoKI: never overlaps —no effect
  • EcoBI: never overlaps —no effect

Digestion of Agarose-embedded DNA

  • Minimum 5units of the enzyme are required for complete digestion of 1μg of agarose-embedded lambda DNA in 16hours

Compatible Ends

  • Bsp119I, Bsu15I, Hin1I, Hin6I, HpaII, MaeII, MspI, NarI, Psp1406I, SsiI, XmiI