pUC19 DNA is digested to completion with the appropriate Fermentas PureExtreme restriction enzyme, then purified and dissolved in storage buffer. The DNA fragments contain blunt or sticky ends, depending on the restriction enzyme used for the marker's preparation. pUC19 DNA/MspI (HpaII) Marker can be labeled radioactively with T4 Polynucleotide Kinase. Alternatively it can be labeled radioactively or non-radioactively with Klenow Fragment or Klenow Fragment, exo- with the fill-in reaction.
Sizing and approximate quantification of small DNA fragments