Thermo Scientific™ PstI

The PstI restriction enzyme recognizes CTGCA^G sites and cuts best at 37°C in O buffer (Isoschizomers: BspMAI).

Overview
Brand: Thermo Scientific™

Manufacturer Part Number: ER0612

PSTI 10U/UL 5X3000U

UNSPSC: 12352204

Code: 88

Additional Details:
Additional Details: Weight: 0.02000kg



Product Code. 10293110

Quantity Price
1 £ 62.75 / Pack of 5
EU Stock 8
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Description and Specification

Specification

Concentration 10U/µL
Product Name PstI
Units 5 x 3,000 U
Product Name PstI
Item Description 10U/uL, 5x3000U

5'..C T G C A▵G...3'

3'..G▵A C G T C...5'

Conditions for 100% Activity

  • 1X Buffer O:50mM Tris-HCl (pH 7.5 at 37°C), 10mM MgCl2, 100mM NaCl and 0.1mg/mL BSA
  • Incubate at 37°C

Storage Buffer

  • PstI is supplied in: 10mM Tris-HCl (pH7.4 at 25°C), 200mM NaCl, 1mM DTT, 0.1mM EDTA, 0.15% Triton X-100, 0.2mg/mL BSA and 50% (v/v) glycerol

Ligation and Recleavage

  • After 50-fold overdigestion with PstI, more than 95% of the DNA fragments can be ligated and recut

Methylation Effects

  • Dam: never overlaps —no effect
  • Dcm: never overlaps —no effect
  • CpG: never overlaps —no effect
  • EcoKI: never overlaps —no effect
  • EcoBI: never overlaps —no effect

Digestion of Agarose-embedded DNA

  • Minimum 5units of the enzyme are required for complete digestion of 1μg of agarose-embedded lambda DNA in 16hours

Compatible Ends

  • Alw21I, ApaI, BseSI, Eco24I, Mph1103I, PstI, SacI, SdaI

Note:

Conditions of high pH, low salt, high glycerol, 8% DMSO can cause star activity (Malyguine, E., et al., Gene, 8, 163-177, 1980). Surrounding sequences: the presence of adjacent runs of G-C base pairs confers significant resistance to cleavage (Armstrong, K. and Bauer, W.R., NAR, 10, 993-1007, 1982). 100% dUTP incorporation at the recognition site reduces PstI cleavage to 25% (Glenn, T.C., et al., Biotechniques, 17, 1086-1090, 1994). PstI will not cut AGCTGCAG when methylated by AluI methyltransferase.