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Thermo Scientific™ PdmI (XmnI)

The PdmI (XmnI) restriction enzyme recognizes GAANN^NNTTC sites and cuts best at 37°C in Tango buffer (Isoschizomers: Asp700I, MroXI, XmnI).

Brand:  Thermo Scientific™ ER1531

UNSPSC : 12352204

Code : 88

Additional Details : Weight : 1.51950kg

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Product Code. 10212740

  • £28.12 / 500 units


EU Stock 15
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Specifications

Description

5'...G A A N N▵N N T T C...3'

3'...C T T N N▵N N A A G...5'

Conditions for 100% Activity:

  • 1X Buffer Tango: 33mM Tris-acetate (pH 7.9 at 37°C), 10mM Mg-acetate, 66mM K-acetate and 0.1mg/mL BSA
  • Incubate at 37°C

Storage Buffer:

  • PdmI is supplied in: 10mM Tris-HCl (pH 7.5 at 25°C), 100mM KCl, 1mM DTT, 5mM MgCl2, 0.2mg/mL BSA and 50% (v/v) glycerol

Ligation and Recleavage:

  • After 50-fold overdigestion with PdmI, more than 95% of the DNA fragments can be ligated and recut

Methylation Effects:

  • Dam: never overlaps —no effect
  • Dcm: never overlaps —no effect
  • CpG: may overlap —cleavage impaired
  • EcoKI: may overlap —effect not determined
  • EcoBI: may overlap —effect not determined

Digestion of Agarose-embedded DNA:

  • Minimum 10units of the enzyme are required for complete digestion of 1μg of agarose-embedded lambda DNA in 16 hours
Description & Specifications

Specifications

PdmI (XmnI)
10U/µL
500U