missing translation for 'onlineSavingsMsg'
Learn More

Thermo Scientific™ PauI (BssHII)

The PauI (BssHII) restriction enzyme recognizes G^CGCGC sites and cuts best at 37°C in R buffer (Isoschizomers: BsePI, BssHII).

Brand:  Thermo Scientific™ ER1091

UNSPSC : 12352204

Code : 88

Additional Details : Weight : 0.01000kg

 View more versions of this product

Product Code. 10430100

  • £23.45 / 200 units


Estimated Shipment
08-09-2017
Add to basket

Description

Description

5'...G▵C G C G C...3'

3'...C G C G C▵G...5'

Conditions for 100% Activity:

  • 1X Buffer R: 10mM Tris-HCl (pH 8.5 at 37°C), 10mM MgCl2, 100mM KCl and 0.1mg/mL BSA
  • Incubate at 37°C

Storage Buffer:

  • PauI is supplied in:10mM Tris-HCl (pH 7.5 at 25°C), 50mM KCl, 1mM DTT, 0.1mM EDTA, 0.2mg/mLBSA and 50% (v/v) glycerol

Ligation and Recleavage:

  • After 50-fold overdigestion with PauI, more than 95% of the DNA fragments can be ligated and recut

Methylation Effects:

  • Dam: never overlaps —no effect
  • Dcm: never overlaps —no effect
  • CpG: completely overlaps —blocked
  • EcoKI: never overlaps —no effect
  • EcoBI: never overlaps —no effect

Digestion of Agarose-embedded DNA:

  • Minimum 10units of the enzyme are required for complete digestion of 1μg of agarose-embedded lambda DNA in 16hours

Compatible Ends:

  • AflIII, AscI, BtgI, MluI

Note:

Low salt, high glycerol (>5%) concentrations or a large excess of enzyme may result in star activity.

Specifications

Specifications

PauI (BssHII)
10U/µL
200U