Thermo Scientific™ OliI (AleI)

The OliI (AleI) restriction enzyme recognizes CACNN^NNGTG sites and cuts best at 37°C in R buffer (Isoschizomers: AleI).

Brand: Thermo Scientific™

Manufacturer Part Number: ER1632

OLII (ALEI) 10U/UL 1000U

UNSPSC: 12352204

Code: 88

Additional Details:
Additional Details: Weight: 0.01000kg

Product Code. 10222460

Quantity Price
1 £103.00 / 1000 units
EU Stock 5
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Description and Specification


Product Name OliI (AleI)
Concentration 10U/µL
Units 1,000U

5'...C A C N N▵N N G T G...3'

3'...G T G N N▵N N C A C...5'

Conditions for 100% Activity:

  • 1X Buffer R: 10mM Tris-HCl (pH 8.5 at 37°C), 10mM MgCl2, 100mM KCl and 0.1mg/mL BSA
  • Incubate at 37°C

Storage Buffer:

  • OliI is supplied in: 10mM Tris-HCl (pH 7.4 at 25°C), 100mM KCl, 1mM DTT, 1mM EDTA, 0.2mg/mL BSA and 50% (v/v) glycerol

Ligation and Recleavage:

  • After 50-fold overdigestion with OliI, more than 80% of the DNA fragments can be ligated and more than 90% of these can be recut

Methylation Effects:

  • Dam: never overlaps —no effect
  • Dcm: never overlaps —no effect
  • CpG: may overlap —cleavage impaired
  • EcoKI: may overlap —blocked
  • EcoBI: may overlap —blocked

Digestion of Agarose-embedded DNA:

  • Minimum 5units of the enzyme are required for complete digestion of 1μg of agarose-embedded lambda DNA in 16 hours