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Thermo Scientific™ NotI, HC (50 U/μL)

Product Code. 10591031 Shop All Thermo Scientific Products
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1,500 units
Unit Size:
1500 units
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Product Code. 10591031 Supplier Thermo Scientific™ Supplier No. ER0593

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Description

Thermo Scientific™ Fermentas NotI is available as a FastDigest enzyme which is used for rapid DNA digestion.

Thermo Scientific™ NotI is available as a FastDigest enzyme which is used for rapid DNA digestion, more than 95% of the digested DNA fragments can be ligated and recut at a 5'-termini concentration of 0.5μM after a 50-fold overdigestion (3U/μg DNA x 17 hours) with NotI.

5'..G C▵G G C C G C...3'

3'..C G C C G G▵C G...5'

Conditions for 100% Activity:

  • 1X Buffer O: 50mM Tris-HCl (pH 7.5 at 37°C), 10mM MgCl2, 100mM NaCl and 0.1mg/mL BSA
  • Incubate at 37°C

Storage Buffer:

  • NotI is supplied in: 20mM Tris-HCl (pH 7.8 at 25°C), 100mM NaCl, 0.1mM EDTA, 1mM DTT, 0.02% Triton X-100, 0.2mg/mL BSA and 50% (v/v) glycerol

Ligation and Recleavage:

  • After 50-fold overdigestion with NotI, more than 95% of DNA fragments can be ligated and recut

Methylation Effects:

  • Dam: never overlaps —no effect
  • Dcm: never overlaps —no effect
  • CpG: completely overlaps —blocked
  • EcoKI: never overlaps —no effect
  • EcoBI: never overlaps —no effect

Digestion of Agarose-embedded DNA:

  • Minimum 5units of the enzyme are required for complete digestion of 1μg of agarose-embedded Adenovirus-2 DNA in 16 hours

Compatible Ends:

  • Bsp120I, CfrI, Eco52I

Note:

Assayed using pTZ19RJL2 plasmid DNA digested with BseLI. Supercoiled plasmids may require up to 5-fold more NotI for complete digestion than linear DNAs.

TRUSTED_SUSTAINABILITY

Specifications

Concentration 50 U/μL
Methylation Sensitivity CpG Methylation-Sensitive, Not Dam Methylation-Sensitive, Not Dcm Methylation-Sensitive
Enzyme Not I
Compatible Buffer 10X Buffer O
Sensitive to Heat Inactivation Yes
Optimal Reaction Temperature 37°C
Type IIS RE No
Quantity 1,500 units
Product Type Restriction Enzyme
Research Category Traditional Cloning

For Research Use Only. Not for use in diagnostic procedures.

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