Thermo Scientific™ NotI

The NotI restriction enzyme recognizes GC^GGCCGC sites and cuts best at 37°C in O buffer (Isoschizomers: CciNI).

Overview
Brand: Thermo Scientific™

Manufacturer Part Number: ER0593

RESTRICTION ENZYME, CONVENTIONAL, NOTI, 50U/UL,cuts GC¬GGCCGC at 37°C (supplied with 1mL of 10X

UNSPSC: 12352204

Code: 88

Additional Details:
Additional Details: Weight: 1.51950kg



Product Code. 10591031

Quantity Price
1 £ 129.0 / 1500 units
EU Stock 11
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Description and Specification

Specification

Product Name NotI
Concentration 50U/µL
Units HC; 1,500U

5'..G C▵G G C C G C...3'

3'..C G C C G G▵C G...5'

Conditions for 100% Activity:

  • 1X Buffer O: 50mM Tris-HCl (pH 7.5 at 37°C), 10mM MgCl2, 100mM NaCl and 0.1mg/mL BSA
  • Incubate at 37°C

Storage Buffer:

  • NotI is supplied in: 20mM Tris-HCl (pH 7.8 at 25°C), 100mM NaCl, 0.1mM EDTA, 1mM DTT, 0.02% Triton X-100, 0.2mg/mL BSA and 50% (v/v) glycerol

Ligation and Recleavage:

  • After 50-fold overdigestion with NotI, more than 95% of DNA fragments can be ligated and recut

Methylation Effects:

  • Dam: never overlaps —no effect
  • Dcm: never overlaps —no effect
  • CpG: completely overlaps —blocked
  • EcoKI: never overlaps —no effect
  • EcoBI: never overlaps —no effect

Digestion of Agarose-embedded DNA:

  • Minimum 5units of the enzyme are required for complete digestion of 1μg of agarose-embedded Adenovirus-2 DNA in 16 hours

Compatible Ends:

  • Bsp120I, CfrI, Eco52I

Note:

Assayed using pTZ19RJL2 plasmid DNA digested with BseLI. Supercoiled plasmids may require up to 5-fold more NotI for complete digestion than linear DNAs.