Thermo Scientific™ MspI (HpaII)

The MspI (HpaII) restriction enzyme recognizes C^CGG sites and cuts best at 37°C in Tango buffer.

Overview
Brand: Thermo Scientific™

Manufacturer Part Number: ER0541

MSPI (HPAII) 10U/UL 3000U

UNSPSC: 12352204

Code: 88

Additional Details:
Additional Details: Weight: 1.51950kg



Disclaimers: This product or the use of this product is covered US patent application US20100041739A1 and corresponding counterparts owned by Fermentas. The purchase of this product includes a non-transferable license to use this product for the purchaser's internal research. All other commercial uses of this product, including without limitation product use for diagnostic purposes, resale of product in the original or any modified form or product use in providing commercial services require a separate license from Fermentas.

Product Code. 10339339

Quantity Price
1 £ 33.22 / 3000 units
EU Stock 34
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Description and Specification

Specification

Product Name MspI (HpaII)
Concentration 10U/µL
Units 3,000U

5'...C▵C G G...3'

3'...G G C▵C...5'

Conditions for 100% Activity:

  • 1X Buffer Tango™: 33mM Tris-acetate (pH 7.9 at 37°C), 10mM Mg-acetate, 66mM K-acetate and 0.1mg/mL BSA
  • Incubate at 37°C

Storage Buffer:

  • MspI is supplied in: 10mM potassium phosphate (pH 7.5 at 25°C), 200mM NaCl, 1mM DTT, 1mM EDTA, 0.2mg/mL BSA and 50% (v/v) glycerol

Methylation Effects:

  • Dam: never overlaps —no effect
  • Dcm: never overlaps —no effect
  • CpG: completely overlaps —no effect
  • EcoKI: never overlaps —no effect
  • EcoBI: never overlaps —no effect

Compatible Ends:

  • Bsp119I, Bsu15I, Hin1I, Hin6I, MaeII, NarI, Psp1406I, SsiI, TaqI, XmiI

Note:

MspI is an isoschizomer of HpaII. When the external C in the sequence CCGG is methylated, MspI and HpaII cannot cleave. However, unlike HpaII, MspI can cleave the sequence when the internal C residue is methylated.