Thermo Scientific™ Mph1103I (NsiI)

The Mph1103I (NsiI) restriction enzyme recognizes ATGCA^T sites and cuts best at 37°C in R buffer (Isoschizomers: EcoT22I, NsiI, Zsp2I).

Overview
Brand: Thermo Scientific™

Manufacturer Part Number: ER0731

MPH1103I (NSII) 10U/UL 1000U

UNSPSC: 12352204

Code: 88

Additional Details:
Additional Details: Weight: 1.51950kg



Product Code. 10304720

Quantity Price
1 £ 49.94 / 1000 units
EU Stock 7
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Description and Specification

Specification

Product Name Mph1103I (NsiI)
Concentration 10U/µL
Units 1,000U

5'..A▵T G C A▵T...3'

3'..T▵A C G T▵A...5'

Conditions for 100% Activity:

  • 1X Buffer R: 10mM Tris-HCl (pH 8.5 at 37°C), 10mM MgCl2, 100mM KCl and 0.1mg/mL BSA
  • Incubate at 37°C

Storage Buffer:

  • Mph1103I is supplied in: 10mM Tris-HCl (pH 7.4 at 25°C), 200mM KCl, 1mM DTT, 1mM EDTA, 0.2mg/mL BSA and 50% (v/v) glycerol

Methylation Effects:

  • Dam: never overlaps —no effect
  • Dcm: never overlaps —no effect
  • CpG: never overlaps —no effect
  • EcoKI: never overlaps —no effect
  • EcoBI: may overlap —effect not determined

Compatible Ends:

  • Alw21I, PstI, SdaI, SduI