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Thermo Scientific™ MluI

The MluI restriction enzyme recognizes A^CGCGT sites and cuts best at 37°C in R buffer.

Brand:  Thermo Scientific™ ER0562

Code : 88

Additional Details : Weight : 1.51950kg

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Product Code. 10141360

  • £289.28 / 5000 units

EU Stock 2
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5'...A▵C G C G T...3'

3'...T G C G C▵A...5'

Conditions for 100% Activity:

  • 1X Buffer R: 10mM Tris-HCl (pH 8.5 at 37°C), 10mM MgCl2, 100mM KCl and 0.1mg/mL BSA
  • Incubate at 37°C

Storage Buffer:

  • MluI is supplied in: 10mM Tris-HCl (pH 7.5 at 25°C), 50mM KCl, 1mM DTT, 0.1mM EDTA, 0.2mg/mL BSA and 50% (v/v) glycerol

Methylation Effects:

  • Dam: never overlaps —no effect
  • Dcm: never overlaps —no effect
  • CpG: completely overlaps —blocked
  • EcoKI: may overlap —no effect
  • EcoBI: may overlap —no effect

Digestion of Agarose-embedded DNA:

  • Minimum 5U of the enzyme are required for complete digestion of 1μg of agarose-embedded lambda DNA in 16 hours

Compatible Ends:

  • AflIII, BtgI, PauI, SgsI