Thermo Scientific™ MlsI (MscI)

The MlsI (MscI) restriction enzyme recognizes TGG^CCA sites and cuts best at 37°C in R buffer (Isoschizomers: BalI, MluNI, MscI, Msp20I).

Overview
Brand: Thermo Scientific™

Manufacturer Part Number: ER1212

RESTRICTION ENZYME, CONVENTIONAL, MLSI (MSCI),5u/ul, cuts TGG¬CCA at 37°C (supplied with 1mL of

UNSPSC: 12352204

Code: 88

Additional Details:
Additional Details: Weight: 0.01000kg



Product Code. 10283160

Quantity Price
1 £ 129.0 / 1000 units
EU Stock 5
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Description and Specification

Specification

Product Name MlsI (MscI)
Concentration 5U/µL
Units 1,000U

5'..T G G▵C C A...3'

3'..A C C▵G G T...5'

Conditions for 100% Activity:

  • 1X Buffer R: 10mM Tris-HCl (pH 8.5 at 37°C), 10mM MgCl2, 100mM KCl and 0.1mg/mL BSA
  • Incubate at 37°C

Storage Buffer:

  • MlsI is supplied in: 10mM Tris-HCl (pH 7.4 at 25°C), 100mM KCl, 1mM DTT, 1mM EDTA, 0.2mg/mL BSA and 50% (v/v) glycerol

Methylation Effects:

  • Dam: never overlaps —no effect
  • Dcm: may overlap —blocked
  • CpG: never overlaps —no effect
  • EcoKI: never overlaps —no effect
  • EcoBI: never overlaps —no effect

Digestion of Agarose-embedded DNA:

  • Minimum 20U of the enzyme are required for complete digestion of 1μg of agarose-embedded lambda DNA in 16 hours

Note:

Assayed using lambda DNA (dcm-) (#SD0021). MlsI is blocked by overlappingdcm methylation. To avoiddcm methylation, use adam-, dcm strain such as GM2163 (#M0099).