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Thermo Scientific™ MboII

The MboII restriction enzyme recognizes GAAGA(8/7)^ sites and cuts best at 37°C in B buffer.

Brand:  Thermo Scientific™ ER0822

Code : 88

Additional Details : Weight : 0.01000kg

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Product Code. 10394770

  • £142.00 / 1500 units


EU Stock 3
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Description

Description

5'...G A A G A (N)8▵...3'

3'...C T T C T (N)7▵...5'

Conditions for 100% Activity:

  • 1X Buffer B: 10mM Tris-HCl (pH 7.5 at 37°C), 10mM MgCl2 and 0.1mg/mL BSA
  • Incubate at 37°C

Storage Buffer:

  • MboII is supplied in: 10mM Tris-HCl (pH 7.5 at 25°C), 50mM KCl, 1mM DTT, 0.1mM EDTA, 0.2mg/mL BSA and 50% (v/v) glycerol

Methylation Effects:

  • Dam: may overlap —blocked
  • Dcm: never overlaps —no effect
  • CpG: may overlap —no effect
  • EcoKI: never overlaps —no effect
  • EcoBI: may overlap —no effect

Note:

Assayed using lambda DNA (dam) (#SD0021). MboII is blocked by overlappingdam methylation. To avoiddam methylation, use adam,dcm- strain such as GM2163 (#M0099). Greater than 15-fold overdigestion with MboII may result in star activity. MboII produces DNA fragments that have a single-base 3 extension which are more difficult to ligate than blunt-ended fragments. MboII may remain associated with the cleaved DNA. This may cause DNA band shifting during electrophoresis. To avoid atypical DNA band patterns, use the 6X DNA Loading Dye & SDS Solution for sample preparation or heat the digested DNA in the presence of SDS prior to electrophoresis.

Specifications

Specifications

MboII
5U/µL
1,500U
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