Thermo Scientific™ Maxima Probe/ROX qPCR Master Mix (2X)

Product Code. 11813923

Brand: Thermo Scientific™ K0232

Description

Optimize qPCR with probe chemistry using standard cycling protocols with these ready-to-use qPCR master mixes.

Maxima Probe qPCR Master Mixes are ready-to-use solutions optimized for quantitative real-time PCR. The master mixes include Maxima Hot Start Taq DNA Polymerase and dNTPs in an optimized PCR buffer. Only template and primers need to be added.

Maxima Hot Start Taq DNA Polymerase in combination with an optimized buffer ensures PCR specificity and sensitivity. dUTP is included in the mix for optional carry-over contamination control using uracil DNA glycosylase (UDG). The use of Maxima Probe qPCR Master Mixes in real-time PCR ensures reproducible, sensitive and specific quantification of genomic, plasmid, viral, and cDNA templates.

Maxima Probe qPCR Master Mixes are compatible with most real-time thermal cyclers.

The master mix includes Maxima Hot Start Taq DNA Polymerase and dNTPs in an optimized PCR buffer. It is supplemented with ROX passive reference dye. Only template and primers need to be added.Maxima Hot Start Taq DNA Polymerase in combination with an optimized buffer ensures PCR specificity and sensitivity. dUTP is included in the mix for optional carryover contamination control using uracil DNA glycosylase (UDG). ROX serves as a passive reference dye. The use of Maxima Probe/ROX qPCR Master Mix (2X) in real time PCR ensures reproducible, sensitive and specific quantification of genomic, plasmid, viral and cDNA templates. The master mix can be used with most real-time thermal cyclers, including instruments from Applied Biosystems, Eppendorf, Corbett, Bio-Rad and Roche.

• Sensitivity—detects low copy number targets
• Specificity—Maxima Hot Start Taq DNA Polymerase and the optimized buffer eliminate non-specific amplification and formation of primer dimers
• Wide linear range—accurate quantification across 9 orders of magnitude
• Universal—can be used with sequence-specific probes on most real-time thermal cyclers
• Reproducibility and convenience—ready-to-use 2X master mix minimizes pipetting error and reduces set-up time

Recommended for:

Real-time PCR using sequence-specific probes; Real-time RT-PCR using sequence-specific probes.

Specifications

• Concentration: 2X
• Content And Storage: Contains:
  • 10 x 1.25 mL Maxima Probe/ROX qPCR Master Mix (2X) containing Maxima Hot Start Taq DNA Polymerase and dNTPs (also dUTP) in an optimized PCR buffer with ROX passive reference dye; sufficient for 1000 x 25 μL reactions.
  • 10 x 1.25 mL Nuclease-Free Water
  
  Store at -20°C.
• Detection Method: Primer-probe
• Format: Tube
• GC-Rich PCR Performance: High
• PCR Method: qPCR
• Polymerase: Taq DNA Polymerase
• Reaction Speed: Standard
• For Use With (Equipment): 7000 System, 7300 System, 7700 System, 7900HT System, StepOnePlus™ System, StepOne™ System
• Product Line: Maxima
• Product Type: qPCR Master Mix
• Quantity: 1,000 reactions
• Sufficient For: 1000 Reactions at 25 μL
• For Use With (Application): Gene Expression
• No. of Reactions: 1,000 Reactions
• Sample Type: DNA, dsDNA
• Volume: 10 x 1.25 mL

For Research Use Only. Not for use in diagnostic procedures.