Thermo Scientific™ KspAI (HpaI) (10 U/μL)

Artikelnummer.	Quantity	unitSize
10509130	500 units	500 units
10354820	2,500 units	2500 units

Artikelnummer. 10509130 Lieferant Thermo Scientific™ Lieferanten-Nr. ER1031

Beschreibung

The KspAI (HpaI) restriction enzyme recognizes GTT^AAC sites and cuts best at 37°C in B buffer (Isoschizomers: HpaI).

Lambda DNA digested with KspAI (HpaI), 0.7% agarose, 14 cleavage sites

conventional restriction endonucleases are a large collection of high quality restriction enzymes, optimized to work in one of the buffers of the Five Buffer System. In addition, the universal Tango buffer is provided for convenience in double digestions. All of the enzymes exhibit 100% activity in the recommended buffer and reaction conditions. To ensure consistent performance, Thermo Scientific restriction enzyme reaction buffers contain premixed BSA, which enhances the stability of many enzymes and binds contaminants that may be present in DNA preparations.

5'..G T T▵A A C...3'

3'..C A A▵T T G...5'

Note:

High glycerol (>5%) concentration, pH>8.0 or a large excess of enzyme may result in star activity.

Spezifikation

• Concentration: 10 U/μL
• Methylation Sensitivity: CpG Methylation-Sensitive, Not Dam Methylation-Sensitive, Not Dcm Methylation-Sensitive
• Enzyme: KspAI (HpaI)
• Compatible Buffer: 10X Buffer B
• Sensitive to Heat Inactivation: Yes
• Optimal Reaction Temperature: 37°C
• Type IIS RE: No
• Quantity: 500 units
• Product Type: Restriction Enzyme
• Research Category: Traditional Cloning

For Research Use Only. Not for use in diagnostic procedures.