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Thermo Scientific™ KpnI, HC (50 U/μL)

Product Code. 10785831 Shop All Thermo Scientific Products
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Quantity:
20,000 units
Unit Size:
20000 units
This item is not returnable. View return policy

Product Code. 10785831

Brand: Thermo Scientific™ ER0523

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This item is not returnable. View return policy

Description

Thermo Scientific™ Fermentas KpnI is available as a FastDigest enzyme which is used for rapid DNA digestion.

Thermo Scientific™ KpnI is available as a FastDigest enzyme which is used for rapid DNA digestion. More than 95% of the digested DNA fragments can be ligated and recut at a 5'-termini concentration of 0.02μM after a 50-fold overdigestion (3U/μg DNA x 17 hours) with KpnI.

5'..G G T A C▵C...3'

3'..C▵C A T G G...5'

Conditions for 100% Activity:

  • 1X Buffer KpnI: 10mM Tris-HCl (pH 7.5 at 37°C), 10mM MgCl2, 0.02% Triton X-100 and 0.1mg/mL BSA
  • Incubate at 37°C

Storage Buffer:

  • KpnI is supplied in: 10mM Tris-HCl (pH 7.5 at 25°C), 50mM KCl, 1mM DTT, 0.1mM EDTA, 0.2mg/mL BSA and 50% (v/v) glycerol

Methylation Effects:

  • Dam: never overlaps —no effect
  • Dcm: may overlap —no effect
  • CpG: may overlap —no effect
  • EcoKI: never overlaps —no effect
  • EcoBI: never overlaps —no effect

Digestion of Agarose-embedded DNA:

  • Minimum 5U of the enzyme are required for complete digestion of 1μg of agarose-embedded lambda DNA in 16 hours

Note:

High glycerol (>5%) concentrations, pH>8.0 or a large excess of enzyme may result in star activity.

TRUSTED_SUSTAINABILITY

Specifications

Concentration 50 U/μL
Methylation Sensitivity Not Dam Methylation-Sensitive, Not Dcm Methylation-Sensitive, Not CpG Methylation-Sensitive
Enzyme Kpn I
Compatible Buffer Unique Buffer (10X Buffer KpnI)
Sensitive to Heat Inactivation Yes
Optimal Reaction Temperature 37°C
Type IIS RE No
Quantity 20,000 units
Product Type Restriction Enzyme
Research Category Traditional Cloning

For Research Use Only. Not for use in diagnostic procedures.

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