Thermo Scientific™ KpnI, HC (50 U/μL)

Thermo Scientific™ Fermentas KpnI is available as a FastDigest enzyme which is used for rapid DNA digestion.

Brand:  Thermo Scientific™ ER0523

Product Code. 10785831

Description

Thermo Scientific™ KpnI is available as a FastDigest enzyme which is used for rapid DNA digestion. More than 95% of the digested DNA fragments can be ligated and recut at a 5'-termini concentration of 0.02μM after a 50-fold overdigestion (3U/μg DNA x 17 hours) with KpnI.

5'..G G T A C▵C...3'

3'..C▵C A T G G...5'

Conditions for 100% Activity:

• 1X Buffer KpnI: 10mM Tris-HCl (pH 7.5 at 37°C), 10mM MgCl₂, 0.02% Triton X-100 and 0.1mg/mL BSA
• Incubate at 37°C

Storage Buffer:

• KpnI is supplied in: 10mM Tris-HCl (pH 7.5 at 25°C), 50mM KCl, 1mM DTT, 0.1mM EDTA, 0.2mg/mL BSA and 50% (v/v) glycerol

Methylation Effects:

• Dam: never overlaps —no effect
• Dcm: may overlap —no effect
• CpG: may overlap —no effect
• EcoKI: never overlaps —no effect
• EcoBI: never overlaps —no effect

Digestion of Agarose-embedded DNA:

• Minimum 5U of the enzyme are required for complete digestion of 1μg of agarose-embedded lambda DNA in 16 hours

Note:

High glycerol (>5%) concentrations, pH>8.0 or a large excess of enzyme may result in star activity.

Specifications

• Concentration: 50 U/μL
• Enzyme: Kpn I
• Sensitive to Heat Inactivation: Yes
• Type IIS RE: No
• Product Type: Restriction Enzyme

• Methylation Sensitivity: Not Dam Methylation-Sensitive, Not Dcm Methylation-Sensitive, Not CpG Methylation-Sensitive
• Compatible Buffer: Unique Buffer (10X Buffer KpnI)
• Optimal Reaction Temperature: 37°C
• Quantity: HC; 20,000 U
• Research Category: Traditional Cloning

For Research Use Only. Not for use in diagnostic procedures.