Thermo Scientific™ KpnI

The KpnI restriction enzyme recognizes GGTAC^C sites and cuts best at 37°C in its own unique buffer (Isoschizomers: Asp718I).

Brand: Thermo Scientific™

Manufacturer Part Number: ER0521

KPNI 10U/UL 4000U

UNSPSC: 12352204

Code: 88

Additional Details:
Additional Details: Weight: 0.01000kg

Product Code. 10328759

Quantity Price
1 £38.49 / 4000 units
EU Stock 21
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Description and Specification


Product Name KpnI
Concentration 10U/µL
Units 4,000U

5'..G G T A C▵C...3'

3'..C▵C A T G G...5'

Conditions for 100% Activity:

  • 1X Buffer KpnI: 10mM Tris-HCl (pH 7.5 at 37°C), 10mM MgCl2, 0.02% Triton X-100 and 0.1mg/mL BSA
  • Incubate at 37°C

Storage Buffer:

  • KpnI is supplied in: 10mM Tris-HCl (pH 7.5 at 25°C), 50mM KCl, 1mM DTT, 0.1mM EDTA, 0.2mg/mL BSA and 50% (v/v) glycerol

Methylation Effects:

  • Dam: never overlaps —no effect
  • Dcm: may overlap —no effect
  • CpG: may overlap —no effect
  • EcoKI: never overlaps —no effect
  • EcoBI: never overlaps —no effect

Digestion of Agarose-embedded DNA:

  • Minimum 5U of the enzyme are required for complete digestion of 1μg of agarose-embedded lambda DNA in 16 hours


High glycerol (>5%) concentrations, pH>8.0 or a large excess of enzyme may result in star activity.