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Thermo Scientific™ KpnI

The KpnI restriction enzyme recognizes GGTAC^C sites and cuts best at 37°C in its own unique buffer (Isoschizomers: Asp718I).

Brand:  Thermo Scientific™ ER0521

UNSPSC : 12352204

Code : 88

Additional Details : Weight : 1.51950kg

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Product Code. 10328759

  • £45.95 / 4000 units


EU Stock 25
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Description

Description

5'..G G T A C▵C...3'

3'..C▵C A T G G...5'

Conditions for 100% Activity:

  • 1X Buffer KpnI: 10mM Tris-HCl (pH 7.5 at 37°C), 10mM MgCl2, 0.02% Triton X-100 and 0.1mg/mL BSA
  • Incubate at 37°C

Storage Buffer:

  • KpnI is supplied in: 10mM Tris-HCl (pH 7.5 at 25°C), 50mM KCl, 1mM DTT, 0.1mM EDTA, 0.2mg/mL BSA and 50% (v/v) glycerol

Methylation Effects:

  • Dam: never overlaps —no effect
  • Dcm: may overlap —no effect
  • CpG: may overlap —no effect
  • EcoKI: never overlaps —no effect
  • EcoBI: never overlaps —no effect

Digestion of Agarose-embedded DNA:

  • Minimum 5U of the enzyme are required for complete digestion of 1μg of agarose-embedded lambda DNA in 16 hours

Note:

High glycerol (>5%) concentrations, pH>8.0 or a large excess of enzyme may result in star activity.

Specifications

Specifications

KpnI
10U/µL
4,000U