Thermo Scientific™ Pfu DNA Polymerase

Product Code. 10631231

Brand: Thermo Scientific™ EP0571

Description

Optimize PCR applications requiring high fidelity with this highly thermostable DNA polymerase.

Pfu DNA Polymerase is a highly thermostable DNA polymerase from the hyperthermophilic archaeum Pyrococcus furiosus. The enzyme catalyzes the template-dependent polymerization of nucleotides into duplex DNA in the 5’→3’ direction. Pfu DNA Polymerase also exhibits 3’→5’ exonuclease (proofreading) activity that enables the polymerase to correct nucleotide incorporation errors. It has no 5’→3’ exonuclease activity.

Highlights

• Eight times more accurate than Taq DNA polymerase
• Highly thermostable – remains 95% active after 2 hours incubation at 95°C
• Generates blunt-end PCR products
• Incorporates modified nucleotides (e.g., biotin-, digoxigenin-, fluorescently-labeled nucleotides)
• Increased PCR yields with native Pfu DNA Polymerase

Specifications

• Description: Pfu DNA Polymerase
• Quantity: 100U
• For Use With (Application): High-fidelity PCR, Standard PCR, Cloning, RT-PCR, Site-directed Mutagenesis
• Thermostability: remains 95% active after 2 hours incubation at 95°C
• Includes: 0.6mL 10X Pfu Buffer with (NH4)2SO2, 0.60mL 10X Pfu Buffer with MgSO4 and 0.06mL 25mM MgSO4