Thermo Scientific™ Pfu DNA Polymerase

Product Code.	Description	Quantity	unitSize
10631231	Pfu DNA Polymerase	100U	100 units
10180690	Pfu DNA Polymerase	500U	500 units
10339879	Pfu DNA Polymerase (recombinant)	100U	100 units

Product Code. 10631231 Supplier Thermo Scientific™ Supplier No. EP0571

Description

Optimize PCR applications requiring high fidelity with this highly thermostable DNA polymerase.

Pfu DNA Polymerase is a highly thermostable DNA polymerase from the hyperthermophilic archaeum Pyrococcus furiosus. The enzyme catalyzes the template-dependent polymerization of nucleotides into duplex DNA in the 5’→3’ direction. Pfu DNA Polymerase also exhibits 3’→5’ exonuclease (proofreading) activity that enables the polymerase to correct nucleotide incorporation errors. It has no 5’→3’ exonuclease activity.

Highlights

• Eight times more accurate than Taq DNA polymerase
• Highly thermostable – remains 95% active after 2 hours incubation at 95°C
• Generates blunt-end PCR products
• Incorporates modified nucleotides (e.g., biotin-, digoxigenin-, fluorescently-labeled nucleotides)
• Increased PCR yields with native Pfu DNA Polymerase

Specifications

• Description: Pfu DNA Polymerase
• Quantity: 100U
• For Use With (Application): High-fidelity PCR, Standard PCR, Cloning, RT-PCR, Site-directed Mutagenesis
• Thermostability: remains 95% active after 2 hours incubation at 95°C
• Includes: 0.6mL 10X Pfu Buffer with (NH4)2SO2, 0.60mL 10X Pfu Buffer with MgSO4 and 0.06mL 25mM MgSO4