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Thermo Scientific™ HpyF10VI (MwoI)

The HpyF10VI (MwoI) restriction enzyme recognizes GCNNNNN^NNGC sites and cuts best at 37°C in Tango buffer (Isoschizomers: BstMWI, MwoI).

Brand:  Thermo Scientific™ ER1732

Code : A0

Additional Details : Weight : 0.00900kg

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Product Code. 10339709

  • £166.00 / 1500 units


EU Stock 7
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Description

Description

5'..G C N N N N N▵N N G C...3'

3'..C G N N▵N N N N N C G...5'

Conditions for 100% Activity:

  • 1X Buffer Tango™: 33mM Tris-acetate (pH 7.9 at 37°C), 10mM Mg-acetate, 66mM K-acetate and 0.1mg/mL BSA
  • Incubate at 37°C

Storage Buffer:

  • HpyF10VI is supplied in: 10mM Tris-HCl (pH 7.4 at 25°C), 100mM NaCl, 1mM DTT, 1mM EDTA, 0.2mg/mL BSA and 50% (v/v) glycerol

Methylation Effects:

  • Dam: never overlaps —no effect
  • Dcm: never overlaps —no effect
  • CpG: may overlap —cleavage impaired
  • EcoKI: never overlaps —no effect
  • EcoBI: never overlaps —no effect
Specifications

Specifications

HpyF10VI (MwoI)
10U/μL
1500U